Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Kinetics of Tyrosinase Catalyzing Reaction Activated by 3-O-Caffeoylquinic Acid

Authors
Shengzhao Gong, Qingsheng Chen, Shimei Li
Corresponding Author
Shengzhao Gong
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.31How to use a DOI?
Keywords
Tyrosinase, 3-O-caffeoylquinic acid, Activation; Kinetics.
Abstract
The effect of 3-O-caffeoylquinic acid on the monophenolase and diphenolase activity was tested. The experimental results showed that the activation effected of 3-O-caffeoylquinic acid on both monophenolase and diphenolase activity .3-O-caffeoylquinic acid concentration leading 50% activity increase (AC50) was 10.50mg/L for monophenolase and 1.10g/L for diphenolase. 3-O-caffeoylquinic acid can remove the lag time of monophenolase. The activation kinetics of 3-O-caffeoylquinic acid analyzed by Lineweaver-Burk plots demonstrated a competitive activation for the oxidation of L-DOPA and the activation constants (KA) was determined to be 1.12g/L.
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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.31How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Shengzhao Gong
AU  - Qingsheng Chen
AU  - Shimei Li
PY  - 2016/07
DA  - 2016/07
TI  - Kinetics of Tyrosinase Catalyzing Reaction Activated by 3-O-Caffeoylquinic Acid
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.31
DO  - https://doi.org/10.2991/bbe-16.2016.31
ID  - Gong2016/07
ER  -