Temporal Expression MicroRNA-21 in Serum of Patients with Spinal Cord Injury
Hongyan Li, Dongxu Zhao, Minglei Zhang
Available Online July 2016.
- https://doi.org/10.2991/bbe-16.2016.20How to use a DOI?
- Spinal cord injury, Serum, MicroRNA, MicroRNA-21
- Objective: To investigate the levels of microRNA-21 (miR-21) in the serum of patients with spinal cord injury (SCI) and to determine whether there was a correlation with degree of injury. Methods: Quantitative real-time reverse transcription–polymerase chain reaction( q-RT-PCR)was used to measure the serum levels ofmiR-21 at 1, 7 and 28 days and 3 months after SCI 42 in male and female SCI patients (n = 48) and compare with age and sex-matched patients with non spinal cord injuries (NSCI, n = 60) and healthy volunteers (n = 70). Correlations between serum miR-21 levels and age, sex and degree of injury were investigated. Results: The data from the present study show that the serum level of miR-21 immediately increased and peaked on day 7 post-SCI and then declined to the control level. There were no differences between NSCI group and normal control group at each time point. The expression level o fmiR-21 was related to the lesion degree of SCI. The age and sex of the patients did not influence miR-21 expression after SCI. Conclusions: These finding might provide reference for diagnosis and treatment and contribute to the identification of selective and temporal drug targeted therapy after SCI.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Hongyan Li AU - Dongxu Zhao AU - Minglei Zhang PY - 2016/07 DA - 2016/07 TI - Temporal Expression MicroRNA-21 in Serum of Patients with Spinal Cord Injury PB - Atlantis Press SP - 116 EP - 122 SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.20 DO - https://doi.org/10.2991/bbe-16.2016.20 ID - Li2016/07 ER -