Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens
Yi Zhang, Lixue Wen, Haiyan Bao, Yingying Nie, Yan Feng, Zhilong Xiu
Available Online July 2016.
- https://doi.org/10.2991/bbe-16.2016.40How to use a DOI?
- Marine fungus, Dielectric barrier discharge plasma, Chemical mutagens, Secondary metabolic fingerprints.
- To activate cryptic secondary metabolic genes is a crucial issue in the search for new natural products from microorganisms. For this purpose, in the present study, a marine fungal strain Penicillium chrysogenum WP-1 was treated with dielectric barrier discharge plasma, chemical mutagens, and their combination. Seven mutants with higher antibacterial activities were chosen among the colonies that survived the different treatments. As revealed by high resolution LC-MS analysis, their secondary metabolic fingerprints displayed significant variations in the numbers of total distinguishable peaks (metabolites), newly generated metabolites, nitrogenated compounds, compounds with skeletons larger than C20, compounds with molecular weights greater than 400 Da, and in the yields of the original metabolites. Additionally, different mutagenesis treatments resulted in different metabolite profiles. Moreover, most of these mutants showed morphological variations compared with the original strain. This study reveals that the plasma and plasma-chemical mutagen combination are effective methodsto activate silent fungalsecondary metabolites. It lays the foundation for the application of plasma mutagenesis in marine fungal natural product research.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Yi Zhang AU - Lixue Wen AU - Haiyan Bao AU - Yingying Nie AU - Yan Feng AU - Zhilong Xiu PY - 2016/07 DA - 2016/07 TI - Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens BT - International Conference on Biomedical and Biological Engineering PB - Atlantis Press SP - 238 EP - 246 SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.40 DO - https://doi.org/10.2991/bbe-16.2016.40 ID - Zhang2016/07 ER -