Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Mitochondrial Toxicity of Tenofovir Dipivoxil Fumarate in HK-2 Cells

Authors
Qihui Luo, Junjun Zhao, Xinglei Feng, Zhengli Chen, Wen Zeng, Li Gong, Anchun Cheng, Yubo Shen, Chunmei Zhu
Corresponding Author
Qihui Luo
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.43How to use a DOI?
Keywords
Tenofovir Dipivoxil Fumarate, Hk-2 Cell, Mitochondrial Respiratory Chain Complex, Mitochondrial DNA, Mitochondrial Toxicity.
Abstract
Nucleoside analogs have been shown to have mitochondrial toxicity. In vitro tests can evaluate the mechanisms and severity of the toxicity. Tenofovir dipivoxil fumarate (TDF) is a novel drug for type B hepatitis. In this study, HK-2 cells were used to evaluate the effect of TDF on mitochondrial toxicity in the kidney. HK-2 cells were treated for 9 days in seven treatment groups. This study measured the inhibitory rate of cell proliferation, lactic acid levels, activities of mitochondrial respiratory chain complexes I–III, and mitochondrial DNA content as well as mitochondrial ultrastructure. Results exhibited that rates of cell proliferation, activities of complexes I, III and mitochondrial DNA content were reduced, but lactic acid levels increased in the 125 M TDF group. Mitochondrial ultrastructure was damaged in the 125 M TDF and 62.5 M TDF groups. Thus, the 125 M TDF group exhibited noticeable mitochondrial toxicity, whereas 62.5 M TDF presented weak mitochondrial toxicity, and 31.25 M TDF exhibited no obvious mitochondrial toxicity.
Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)

Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.43How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Qihui Luo
AU  - Junjun Zhao
AU  - Xinglei Feng
AU  - Zhengli Chen
AU  - Wen Zeng
AU  - Li Gong
AU  - Anchun Cheng
AU  - Yubo Shen
AU  - Chunmei Zhu
PY  - 2016/07
DA  - 2016/07
TI  - Mitochondrial Toxicity of Tenofovir Dipivoxil Fumarate in HK-2 Cells
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.43
DO  - https://doi.org/10.2991/bbe-16.2016.43
ID  - Luo2016/07
ER  -