Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection

Authors
Qin TANG, Han ZHANG, Jia-qi HAN, Li -rui LI, Ting-chuan LIU, Jing XU, Gang YU, Yin WANG, Xue-ping YAO, Ze-xiao YANG
Corresponding Author
Qin TANG
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.51How to use a DOI?
Keywords
Equine infectious anemia, RT-PCR, Detection.
Abstract
To develop a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method for quick detecting equine infectious anemia virus (EIAV), according the conserved sequences of EIAV gag gene which published in GenBank, 2 specific RT-PCR primers and 8 extension primers were designed and synthesized. A DNA fragment of EIAV gag gene about 370bp was synthesized in vitro by overlapping PCR, then the RT-PCR method for equine infectious anemia detection was established preliminarily through optimizing the reaction conditions, sensitivity and specificity experiments, and validity test. The results showed that the method for the EIAV has a good specificity and sensitivity, and the reaction product was about 370bp DNA electro phoretic band. This method can detect the EIAV target fragment about 119copies/ L. There are no amplification for the detection of Proteus, Actinobacillus, Salmonella, Escherichia coli, Stenotrophomonasmalt ophilia and Streptococcus type II, and the results showed positive reaction for Equine infectious anemia test antigen detection.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.51How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Qin TANG
AU  - Han ZHANG
AU  - Jia-qi HAN
AU  - Li -rui LI
AU  - Ting-chuan LIU
AU  - Jing XU
AU  - Gang YU
AU  - Yin WANG
AU  - Xue-ping YAO
AU  - Ze-xiao YANG
PY  - 2017/05
DA  - 2017/05
TI  - Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.51
DO  - https://doi.org/10.2991/bbe-17.2017.51
ID  - TANG2017/05
ER  -