Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

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94 articles
Proceedings Article

Basic Research on Real-time Evaluating of Thermal Damage of Tissues by Visible-Near Infrared Spectra

Wei-wei JIA, Li-juan DAI, Xi WANG
The feasibility of evaluation thermal damage in real time by Visible-Near Infrared (V-NIR) spectroscopy is investigated. Thermal damage are produced in fresh porcine livers in vitro by laser (808nm, 5W). The V-NIR spectra (330-1100 nm) at distances of 4, 8 and 12 mm from the center of the damage are...
Proceedings Article

Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone

Jian-wen HU, Meng-meng LIU, Ji-zhong HAN, Bin ZENG
Recombinant follicle stimulating hormone(rFSH), which play an important role in assisted reproductive technologies. However, the availability of a suitable system for the screening high production cell lines from transfected mammal cells for the production of rFSH is limited. The purpose of this study...
Proceedings Article

Extracellular Expression and Catalytic Bioactivity of Optimized Human Aromatase (CYP19) in Pichia Pastoris

Yan-ying HU, Yong-hui ZHONG, Jing ZHEN, Hui-tu ZHANG, Shi-ru JIA, Fu-ping LU, Yu-jie DAI, Xiu-li ZHANG
The fragment encoding aromatase (CYP19) with 45 N-terminal amino acids cut-off was cloned into pPIC9K vector and transformed to the yeast Pichia pastoris. The multi-copy recombinant strain was selected for the production of recombinant aromatase. The catalytic bioactivity of secreted enzyme was further...
Proceedings Article

Anticancer Activity of Oridonin Against Esophageal Cancer Cells Enhanced by Special Electromagnetic Field Treated Water

Jiang PI, Jin-huan JIANG, Hua JIN, Xin WANG, Yuan PENG, Ji-ye CAI
Oridonin, the main pharmacological active substance isolated from Rabdosia Rubescens, has been found to show anti-inflammatory, pro-apoptotic, neurological effects, and excellent anticancer activities. However, the clinical application of oridonin is still limited by its poor solubility. The special...
Proceedings Article

Time-Dependent Cellular Injuries Induced by Calcium Oxalate Monohydrate and Dihydrate Crystals

Jian-Min WANG, Ling-Hong HUANG, Jian-Ming OUYANG
Objective: This study aims to compare the cytotoxicity and adhesion difference between oxalate monohydrate (COM) and dehydrate (COD) crystals and African green monkey kidney epithelial cells (Vero). Methods: Through the detection of cell viability, lactate dehydrogenase (LDH) release amount, expression...
Proceedings Article

Research on Intracranial Pressure Changes of Craniocerebral Injury Caused by Cudgels

Hong-fa CHANG, Zhen-jiang LIU, Peng XIA, Yong-min YU, Dai-qin TAO, Hong-wei LI
Objective To research intracranial pressure change rules of craniocerebral injury through experimentation on animals. Method According to the difference of the hit parts, the animals are divided into three groups to collect the intracranial pressure changes through data collection system andpressure...
Proceedings Article

The Neural Mechanism of Acupuncture Analgesia

Qiu-ling XU, Tao LIU
The acupuncture signal in the uplink conduction, one side through the spinal cord segmental relationship affect the adjacent segments dominated by the skin, visceral activity and adjacent segments of the pain in the introduction; more often up to reach the brain stem, Forebrain and other parts, through...
Proceedings Article

Analysis of Animal Models of Oral Ulcers Based on the Characteristics of Clinical Symptoms of Chinese and Western Medicine

Huan LI, Ming BAI, Yan-yan MIAO, Ming-san MIAO
Objective: To analyze the methods and characteristics of animal model of oral ulcer and the characteristics of Chinese and Western medicine oral ulcer disease anastomosis. Methods: To summarize the characteristics of animal models of oral ulcers, modeling methods, analysis of oral ulcer animal model...
Proceedings Article

Study on Animal Model Characterization of Chinese Medicine

Xu ZHANG, Hui ZHOU, Xuan-xuan ZHU, Yi LIANG, Jia-chen WANG, Yan-yan MIAO, Ming-san MIAO
Characterization can reflect objectivity and represent objective things. With the development of pharmacology of Chinese Medicine in recent years and the deepening of animal model research in Chinese medicine, how to accurately and quickly copy the successful model animals according to the characterization...
Proceedings Article

Modern Analysis of Traditional Chinese Medicine for Chronic Abacterial Prostatitis

Jing LIU, Tan WANG, Huan LI, Ming BAI, Ming-san MIAO
Objective: To explore the therapeutic principles of modern Chinese medicine for chronic prostatitis and the application of TCM in CNP. Procedures and Methods: Analysised the relevant information and literature, to explore the mechanism of Chinese medicine for the treatment of prostatitis, sum up TCM...
Proceedings Article

Researches on the Progress of Antiallergic Traditional Chinese Medicine and Its Mechanism

Xuan WU
With the study of anti-allergy medicine, it proves traditional Chinese medicine has anti-allergic effects and several other functions, such as protecting and stabilizing the target cell membrane (reduce or prevent degranulation, release the allergy mediators, increase the intracellular cAMP levels),...
Proceedings Article

The Significance of Restoring the Traditional Diet of Chinese People from the High Incidence of Modern Diseases

Bao-song LIU, Meng-fan PENG, Yan-yan MIAO, Ming BAI, Ming-San MIAO
From the reform and opening up in 1979 to the year of twenty-first Century, China's economy has been growing rapidly,and great changes have taken place in people's traditional eating habits. Gradually westernized modern diet has become one of the main reasons ,which leading the incidence of many chronic...
Proceedings Article

Thinking of Toxicology in Chinese Medicine

Xu ZHANG, Yan-yan MIAO, Ming-san MIAO, Tian-yuan LIU, Tao ZHANG, Cai-ling FENG
This paper summarizes the research achievements of predecessors in the field of toxicology of Chinese medicine, discusses the new understanding of the toxicity of Chinese medicine from the aspects of the toxicity of Chinese medicine, the application of the toxicity of Chinese medicine and the trend of...
Proceedings Article

Inference of Pure Titanium Micro-arc Oxidation Process on Microstructure of Calcium Phosphate Membrane Layer

Yu-lei LI, Jin-jun TANG, Yue-lai DAI, Li-jun ZHANG, Xiao-yu JIN
In this paper, the growth law of the phosphate and calcium layer in different voltage process was studied. The surface morphology of the film was observed by SEM, and then the distribution of phosphorus and calcium in the film was analyzed. The results show that the higher the voltage, the more uniform...
Proceedings Article

Valvular Heart Disease-Related Feed-Forward Loop Network Reveals Some Biological Features for Disease Development

Feng YANG, Ke LIU, Wei ZENG, Guang-bin WANG, Zheng-wen LI, Ke-li HUANG, Ni-ni RAO
This study is to uncover VHD-related important biological features for understanding molecular of this disease. The system analysis strategy is applied to generate the first VHD-related FFL network. Then, the network topology is mapped into the biological space to refine the biological features of VHD....
Proceedings Article

Research Progress of Plant Calcium Nutrition and Calcium / Cold Signal Interaction

Qing-wen SHI, Qiao-bo SONG, Hui-xin WANG, Chun-ming BAI, Di WU, Qi-ping DONG, Xin CHENG, Xiao-ri HAN, Yi-fei LIU
Calcium, as the second messenger inside plants, is extensively involved in plant response and transduction of various stress signals. Plants shall generate calcium signals of specificity under stress, and this shall give a rise to a series of intracellular physiological and biochemical responses. Calcium...
Proceedings Article

A Study on the Local Electrochemical Processes Inside Cavities

In this paper, a set of experiments scanning real potential variations inside cavities under current control were employed to characterize local electrochemical information. The limiting diffusion current and the critical currents sustaining the supersaturation of dissolved gas and completed oxide film-formation...
Proceedings Article

Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye

Pin-san XU, Zheng-yao ZHANG, Ling-yao LI, Yu-bo LIU
Concern over mercury cytotoxicity inside live specimens has encouraged the development of efficient methods for the in vivo detection of this heavy metal. In this study, by using the previous developed fluorescence dye EPNP (2,6-bis-(4'-peperazino-N'-butyl-1',8'-naphthalimide) dimethylpyridine) as a...
Proceedings Article

Analysis of Animal Models of Alopecia Areata(AA) Based on Clinical Features of Traditional Chinese and Western Medicine

Huan LI, Ming BAI, Yan-yan MIAO, Xiao-yan FANG, Ming-san MIAO
Objective: Analysis of alopecia areata animal model production methods, characteristics and characteristics of Chinese and Western alopecia areata anastomosis. Methods: To summarize the characteristics of the alopecia areata animal model, according to alopecia areata diagnosis and clinical characteristics...
Proceedings Article

The Harm of Fog and Thick Haze to the Body and the Prevention of Chinese Medicine

Le KANG, Yan-yan MIAO, Ming-san MIAO, Zhen-ya HU
In recent years, The situation that China's fog and thick haze pollution is very grim, which has threatened people's health and daily life work. fog and thick haze is a meteorological phenomenon, which is the essence of human activities caused by fine particulate pollution. With the increase in the severity...
Proceedings Article

Characterization of a Serine Carboxypeptidase Hoscp from Holotrichia Oblita Faldermann (Coleoptera: Scarabaeoidea)

Dan ZHAO, Ya-kun ZHANG, Xiao-ping YAN, Wei GUO, Xiao-min LIU
In this report, we have identified a novel serine carboxypeptidase, named HoSCP, from the midgut of a destructive agricultural and landscape pest, Holotrichia oblita. HoSCP cDNA gene was cloned by immunoscreening method. Sequence analysis showed about 49kDa HoSCP consists of 457 amino acid residues with...
Proceedings Article

Photosynthetic Characteristics of Marestail Using PAM Fluorometry

Li-nan GAO
In this work, we used PAM fluorometry to examine photosynthetic characteristics of marestail (Hippuris vulgaris) across a water temperature gradient in three lakes. Three lakes were studied across a gradient in water temperature, with low water temperature conditions in Grass Lake and Arrow Bamboo Lake,...
Proceedings Article

Bioink of Multi-Biomaterials with Tyrosine Reinforcement for 3D Bioprinting of Skin Constructs

Ke YANG, Yong SHI, Hong-bo ZHANG, Rui-xue YIN, Shih-mo YANG, Wen-jun ZHANG
In this paper, a bioink composed of multiple native materials with tyrosine micro-particle reinforcement for 3D bioprinting of skin scaffolds was developed. The rheological property of the developed bioink was optimized for 3D Bioprinting process. The results showed that Tyrosine micro-particle reinforcement...
Proceedings Article

Analyze the Animal Model of Pruritus Based on Clinical Symptoms of Chinese and Western Medicine

Ya-juan WEN, Ming BAI, Ming-san MIAO
Objective: Based on the characteristics of clinical symptoms of pruritus, analyze the relevant animal model, and then propose the evaluation and improvement. Method: According to the clinical diagnostic criteria of pruritus in Chinese and Western medicine, summarize the characteristics and modeling methods...
Proceedings Article

Analysis on the Characteristics of Flower Traditional Chinese Medicine for Preventing and Treating Cerebral Ischemia

Li-ling XIANG, Ming-san MIAO, Ya-juan WEN, Jing-yi QIAO
Objectives: To investigate the characteristics and mechanism of flower traditional Chinese medicine for preventing and treating cerebral ischemia. methods: To summarize active components of flower traditional Chinese medicine on cerebral ischemia model and the characteristics of prevention and treatment...
Proceedings Article

Injury Difference of the Un-Aggregated and Aggregated Calcium Oxalate Monohydrate on Human Kidney Proximal Tubular Epithelial Cells

Li-shan HUANG, Da GUO, Jian-ming OUYANG
Purpose: To study the injury difference of the un-aggregated and aggregated calcium oxalate monohydrate (COM) on human kidney proximal tubular epithelial (HKC) cells, and to discuss the molecular and cellular mechanism of kidney stone formation. Methods: The cell injure by crystals was evaluated by measuring...
Proceedings Article

Injured Human Kidney Proximal Tubular Epithelial Cells Modulate Nucleation and Growth of Calcium Oxalate Crystals

Da GUO, Jian-min WANG, Jian-ming OUYANG
Purpose: The oxidative damage of hydrogen peroxide (H2O2) on human kidney proximal tubular epithelial cell (HKC) and the nucleation and growth of calcium oxalate (CaOx) crystals modulated by normal and injured cells were investigated. Methods: The method of CCK-8 was used to detect the proliferating...
Proceedings Article

Study on Terpenoid Compounds of Different Tissues of Camphor Tree

Ying-ying YUE, Yan FU, Jie CHANG
Based on the current research value of biomass and terpenoids, in this paper, the terpenoids contented in the different organizational of the camphor tree were analyzed by SDE extract method and GC-MS analysis method. The results showed that the contents of terpenoids contained in camphor leaves were...
Proceedings Article

Isolation and Identification of a New Clostridium Butyricum XYB11 Strain Producing 1, 3-Propandiol from Soil

Yan-bo XUE, Miao-miao YANG, Jun-hua YUN, Tinashe Archbold MAGOCHA, Yi-lin QI, Lin-sen YANG, Ming-ming GUO, Fan Zou, Xiang-hui QI
1, 3-propanediol (1,3-PD) is an important chemical material, which is wildly used in many fields. Clostridium butyricumis key strain which can transform glycerol into 1,3-PD. In this study, a new strain producing 1,3-PD was isolated from soil. On the basis of morphology, biochemical-physiological characteristics,...
Proceedings Article

Characterization of Microbial Community Composition in the Fermentation Starter (Daqu) Reveals a Distinct Dominant Species Combination Potentially Contributing to the Flavor of Chinese Gujingtribute Liquor

Hui-min ZHANG, Hong-kui HE, Run-jie CAO, Lei LIU, Zhi-hui TANG, An-jun LI, Zhi-zhou ZHANG
In this study the microbial composition in the starter (Daqu) of Chinese Gujing Tribute liquor was determined by employing near full length ribosome gene library sequencing approach. Gujing Tribute Daqu has a distinct microbial community structure by comparison with several other important types of Chinese...
Proceedings Article

Isolation and Preliminary Molecular Characterization of Novel Anaerobic Microbial Species from the Interface Between Zaopei (Fermented Grains) and the Bottom Pit Mud

Ying HAN, Hong-kui HE, Hang DU, Run-jie CAO, Hai-ning NING, Zhi-hui TANG, An-jun LI, Zhi-zhou ZHANG
Strong-aroma type liquor occupies over 70% of Chinese liquor market, but the namelist of key microbes responsible for liquor flavor is still vague. Lots of data indicate that the interface between the bottom pit mud and Zaopei (fermented grains) plays an important role for aroma molecule production,...
Proceedings Article

Anti-Proliferative Activity of Benzamide Analogs in A549 Tumor Cells in Vitro

Li-wen SHEN, Yan-ling WU, Min CHEN, Yoshimasa Tanaka, Wen ZHANG
Objective: To determine the antitumor activity of five benzamide derivatives with similar structure in A549 tumor cells. Methods: Cytotoxicity of the compounds in A549 tumor cells were monitored through an xCELLigence Real-Time Cell Analysis System. Results: Four benzamide analogs inhibited the growth...
Proceedings Article

Antitumor Effect and Immune Regulation of the Pingyan Capsule on Lewis Lung Cancer Cells in Vivo

Na LI, Yan LI, Tian-yu LIU
Objective: we investigated the function of Pingyan capsule on antitumor and immune regulation in Lewis lung cancer tumor-bearing mice. Methods: mice were randomly divided into normal control group, model group, positive control group, high, medium and low dose Pingyan capsule groups. Lewis lung carcinoma...
Proceedings Article

Identifying the Sulfate Ion Binding Residues in Proteins

Shao-bo LI, Xiu-zhen HU, Li-xia SUN, Xiao-jin ZHANG
Many proteins function execution depends on the process of protein and ligand interact with each other. The identification of ligand binding residues is important for the research of the protein function. The 4442 protein chains with
Proceedings Article

Antimicrobial Activities of Polyphenol Extract of Peanut Skins

Wen-xu XIA, Ping ZHAO, Jia-bin WANG, Zhan-juan LI, Nanju Alice LEE
In this study, the cardinal and purple peanut testa were used as the raw materials for extracting polyphenol substances with ultrasonic and freeze-drying. Then the bacteriostasis and antifungal activities of the extracts were investigated by the methods of disc diffussion and equivalent against the Benzylpenicillin...
Proceedings Article

Effects of Tween 80 on Degradation of Benzo[ƒ]pyrene in Soils Growing Sudan Grass

Sheng-wang PAN, Zhi-hua LEI, Yun-xiao WU, Mao-ping HE
Phytoremediation is becoming a cost-effective technology for the in-situ clean up of sites polluted with hydrophobic organic contaminants (HOCs). The major factors limiting phytoremediation are the mass transfer, rate of plant uptake, and microbial biodegradation of HOCs. To evaluate the potential of...
Proceedings Article

Aqueous Two-Phase System Combined with Ultrasound for the Extraction Of Polysaccharide from Hawthorn (Crataeguspinnatifida) Leaves

Ri-li HAO, Juan-juan MA, Kun LI, Chuan-he ZHU, Xiao-cun ZHANG
Aqueous two-phase system (ATPS) combined with ultrasonic technology was employed to extract polysaccharide from hawthorn leaves in this study. The partition behaviors in different ATPS were investigated first, polyethylene glycol (PEG) 11.5% and (NH4)2SO4 14% were selected as the optimum solvent for...
Proceedings Article

Molecular Actions of Marine Bioactive Compound Fucoidan

Xue-lian WANG, Yan ZHAO
Fucoidan is a complex sulfated polysaccharide mainly found in brown seaweed. It has been used in a wide range of applicationsacross different industries such as food additives, dietary supplements and pharmaceuticals. Studies have shown that fucoidan has numerous biological activities including anti-cancer,...
Proceedings Article

Correlation between Analgesic Effect of Electroacupuncture "ST36" - "GB34" and DNA Methyltransferase on Sciatica Rats Dnmt3a

Qiu-ling XU, Tao LIU
To observationthe relationship between spinal inhibition of DNA methylation in electroacupuncture (EA) analgesia, and spinal cord Dnmt3amRNA expression. EA and inhibitors of 5-AZA Dnmt can improve CCI animals hyperalgesia state and decreased Dnmt3amRNA expression. DNA methylation is closely related to...
Proceedings Article

Analysis of Chronic Skin Ulcer Model Based on Clinical Features of Traditional Chinese Medicine and Western Medicine

Ming-san MIAO, Xiao-li YAN, Ming BAI, Yan-yan MIAO
Objective: Analysis anastomosis of the commonly used animal models and clinical features of chronic skin ulcer disease, put forward to recommendations for model refinement. Method: Summarize the characteristics of existing chronic skin ulcer model and molding method, according to traditional Chinese...
Proceedings Article

ITS andTrnl-F Sequences Analysis of Pulsatilla Chinensis (Bge.) Regel

Shao-xuan ZHANG, Guang-zhu LIN, Tian-tian WANG, Ya-rong LI
To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Pulsatilla chinesis(Bge.) Regel., we extracted the genome DNA from the leaves of Pulsatilla chinesis(Bge.) Regel., collected in Jilin Province, amplified the ITS and trnL-F region using...
Proceedings Article

Ureteroscopy Holmium Laser Lithotripsy Treatment Complicated Ureteral Stones

Fan-ping MENG, Jun WEI, Yong-liang CUI, Ji GAO
Objective: to discuss the effectiveness and safety of the ureteral stone in complicated ureteroscope of ureteroscope. Methods: in this study, 47 cases of ureteral calculi were treated by the ureteroscope of the ureteroscope, which was collected from February 2015 to February 2017. Results: in 47 cases,...
Proceedings Article

Transurethral Plasma Cutting and Prostate Tear in Addition to the Treatment of Hyperplasia of Prostate the Clinical Efficacy of Comparison

Fan-ping MENG, Di ZOU, Yan HE, Ji GAO
Objective To compare the efficacy and complications between bipolar plasmakinetic resection of prostate and bipolar plasmakineticenucleation of prostate for benign prostatic hyperplasia(BPH). Methods 303 cases of BPH were divided into 2 groups, 143 cases aged (73ñ13.4) yearswith prostate volume of (76.5ñ22.5)ml...
Proceedings Article

Soft Renal Calyx Calculi under Ureter Mirror Treatment

Fan-ping MENG, Yan HE, Di ZOU, Ji GAO
Objective: to study the electronic soft ureter mirror joint qin laser lithotripsy in the treatment of renal calyx calculi under safety and effectiveness.Methods: our hospital in March 2016 - March 2017 electronic soft ureter mirror qin laser lithotripsy under 40 patients under the treatment of renal...
Proceedings Article

Design and Development of Long Range Surface Plasmon Resonance Sensor Chip and System for High Sensitivity Detection

Lu-lu ZHANG, Xing CHEN, Cun-fang XU, Yan-fei REN, Ya-ting LI, Da-fu CUI
Long range surface plasmon resonance (LRSPR) was used to improve the propagation lengths, electric field strengths, and the sharpness of angular resonance curves compared with the conventional surface plasmon resonance (CSPR). LRSPR sensor chip was designed with buffer layer whose refractive indices...
Proceedings Article

Effect of Mental Fatigue on Error Monitoring

Feng MA, Yi XIAO, Gui CAI, Feng-gang XU, Shan-guang CHEN
Performance monitoring tasks generate response-locked event-related potentials (ERPs), generated from anterior cingulate cortex (ACC) region of the medial prefrontal cortex. However, the quantitative relationship between mental fatigue and error monitoring has not been closely investigated, which is...
Proceedings Article

Fed-Batch Fermentation of L- Threonine by Escherichia Coli Supplemented with B-Vitamins

Yue-wen SU, Qun-qun GUO, Sen WANG, Xin ZHANG, Jian WANG
B-vitamins can be used as cofactors in the synthesis process of L-threonine. Concentration of the cofactor can effectively change the activity of specific enzymes and reallocate the distribution of carbon substances in the cells. Theeffects of B-vitamins, including calcium pantothenate (VB5), cobalamin...
Proceedings Article

Analysis of Cytoplasm Polymorphism on the TR2 Locus of Mitochondria Genome in Leaf Beet Line SK-5

Qiao-hong LIU, Li-ping LIU, Cheng-fei LUO, Da-you CHENG, Cui-hong DAI, Shu-zhi SHI, Nai-guo LIANG, Tian-jiao LIU
One mitochondrial minisatellite locus was used to study cytoplasmic diversity in leaf beet lines SK-5 population from Chongqing, China. The analysis of one mitochondrial minisatellite locus (TR2) was conducted using variable number of tandem repeats (VNTRs) molecular markers method within 50 single plants...
Proceedings Article

Exercise Related Sudden Death: The Changes of Expression of Bax, Bcl-2 Protain in Myocardial Tissue and Brain Motor Cortex

Yu QIAN, Ren-Jun YANG, Jin YIN
Purposes: Study the changes of cardiac and brain function of exercise related sudden death in rats under over fatigue state and explore the causes of sudden death preliminarily. Methods: Totally 72 male SD rats.7 rats were randomly selected as negative control group. The others were trained continuously...
Proceedings Article

Physiological and Biochemical Effect of Endurance Training of Altitude Training on Fixed Maximal Oxygen Expenditure in Middle and Long Distance Runners

Lang QIN, Jin YIN
The purpose of this study was to investigate the exercise intensity of endurance training of altitude training under the plain and plateau by means of the fixed maximal oxygen expenditure. Eight middle and long distance runners voluntarily participated in this study, the ergmeter bike and telemetry cardiopulmonary...
Proceedings Article

Preliminary Study on a RT-PCR Method for the Equine Infectious Anemia Virus Detection

Qin TANG, Han ZHANG, Jia-qi HAN, Li -rui LI, Ting-chuan LIU, Jing XU, Gang YU, Yin WANG, Xue-ping YAO, Ze-xiao YANG
To develop a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method for quick detecting equine infectious anemia virus (EIAV), according the conserved sequences of EIAV gag gene which published in GenBank, 2 specific RT-PCR primers and 8 extension primers were designed and synthesized. A DNA...
Proceedings Article

One Orthogonal Phase Microscopy Technique for Orthogonal Phase Imaging of a Cell

Jing-ye LIU, Yuan-yuan XU, Qiong ZHU, Meng-yuan SHANG, Jing-rong LIAO, Ya-wei WANG
For human beings, the morphological features of cells are closely related to human health thus research into the technique of cell imaging is very important. The optical phase imaging technique is one of the most useful methods because the cell is a phase object. More than one phase images should be...
Proceedings Article

Direct Electrochemistry of Glucose Oxidase Immobilized on a Hydroxyl Fullerenes- Tio2 Nanocomposite Modified Glassy Carbon Electrode

Di LI, Xiao-Yu ZHANG, Bao-Lin XIAO, Jun HONG, Moosavi-Movahedi Ali Akbar
By immobilizing glucose oxidase (GOD) on a nanocomposite containing hydroxyl fullerenes (C60-OH) and TiO2 nanoparticles, a new type biosensor was prepared for glucose detection. The cyclic voltammograms (CVs) of GOD on the nanocomposite modified glassy carbon electrode (GCE) was quasi-reversible. The...
Proceedings Article

Cloning, Expression and Enzymatic Characterization of Chitin Deacetylase 4 from Hyphantria Cunea (Drury)

Xiao-ping YAN, Dan ZHAO, Ya-kun ZHANG, Wei GUO, Kun-li ZHAO, Wei WANG, Yu-jie GAO
A novel chitin deacetylase (CDA), HcCDA4, was identified from the American white moth, Hyphantria cunea. The full-length cDNA sequence of HcCDA4 was identified,and the cDNA is 1494bp in length. The HcCDA4 was shown as a 56 kDa protein in E. coli BL21 by SDS-PAGE analysis and the specific antibodies reacting...
Proceedings Article

Improved Camptothecin Production of Camptotheca Acuminata on Hydroponic Medium Supplemented with Various Chemicals

Qing-qian ZENG, Lin JIANG, Long-ping ZHU, Hai ZHENG, De-po YANG
A Hydroponic culture was conducted to determine the effect of organic acids and metal ions on plant growth, camptothecin concentrations and yield of Camptotheca acuminata, in an effort to increase the camptothecin yield by artificial cultivation. For maximal camptothecin production, increase of maleic...
Proceedings Article

Review and Outlook of Stem Cell Transplantation for Neonatal Hypoxic- Ischemic Encephalopathy

Qiu-li WANG, Hong-yan LV, Peng-shun REN, Lian-xiang LI
Purpose: To research the states and feasibility of stem cells for neonatal hypoxic ischemic encephalopathy (HIE). Methods: In this study, "newborn, hypoxic ischemic encephalopathy, pathogenesis, stem cell transplantation" as the key words, which are searched the papers published in CNKI database and...
Proceedings Article

Twin-Studies on the Role of Non-Genetic Factors in T1D Pathogenesis

Li-li MA, Yan-ling WU, Ying DONG, Yoshimasa TANAKA, Wen ZHANG
Type 1 diabetes (T1D) is a complex autoimmune disease characterized by T cell-mediated destruction of pancreatic cells, which is resulted from the interaction of genetic and environmental factors. Twin-study is one of the most important methods to identify the genetic basis of complex diseases or traits...
Proceedings Article

Expression of NOB1 Gene and Its Clinical Significance in Ovarian Cancer

Ning LIU, Shun-qing ZHOU, Yang LIN
[Purposes] To explore the correlation between mRNA expression of NOB1 gene in tissues from ovarian cancer, normal ovary, benign ovarian tumor. [Methods] 50 samples of ovarian cancer as the experimental group, 20 samples of normal ovarian tissues and 50 samples of benign ovarian tumor as control groups....
Proceedings Article

Microrna -145 Expression Inhibits Invasion and Metastasis of Cervical Cancer Cells

Shun-qing ZHOU, Ning LIU, Yang LIN
[Purposes] To investigate the expression of microRNA-145 on invasion and metastasis of cervical cancer cells. [Methods] Analysis of the relationship between high expression of microRNA-145 on invasion and metastasis of cervical cancer cells by Transwell invasion assay and Wound scratch assay. [Results]...
Proceedings Article

The Histological Study of Orthodontic Force on the Periodontal Tissues Regenerated by Nano Bioceramics in Beagle Dogs

Lan-lei WANG, He-ying HOU, Sheng-yan YU, Ai-zhong GUAN, Yun-mao LIAO
The aim of the experiment was to study the histological reaction of orthodontic force on the periodontal tissues regenerated by nano biphasic calcium phosphate (NBCP) bioceramics in beagle dogs. Two third incisors were randomly selected and operated as experimental group. The contralateral third incisors...
Proceedings Article

A Food-Grade, Secretory Expression of IL1RA Protein in a Lactococcus Lactis System for Potential Oral Therapy on IBD

Jin CHENG, Jin CAO, Jia CAO, Xiao-feng WANG, Yan-ru GE
Interleukin 1 (IL1 ) is a potential target for IBD treatment. IL1 receptor antagonist (IL1RA) can blocks the biologic activity of IL1 by competitively inhibiting IL1 binding to the IL1R, so IL1RA is suggested to have possible therapeutic applications in treatment of IBD. By using a food-grade recombinant...
Proceedings Article

Development of a Novel Multi-Functional Rehabilitation Robot

Cheng-dong WEI, Quan-quan LIU, Li-hong DUAN, Tong-yang SUN, Meng LI, Zhan ZENG, Chun-bao WANG, Qing SHI, Zhuo-hua LIN, Ya-jing SHEN, Wan-feng SHANG, Zheng-zhi WU
With the development of aging society, the number of hemiplegia patients grows rapidly and over 75% of survivors suffering hemiplegia need rehabilitation training. Traditional training methods need one or more physical therapists to server one patient at the same time. In order to reduce the workload...
Proceedings Article

Development of a FES System for Hemiplegic Rehabilitation Based on Patient Healthy Limb's EMG Signal

Zhan ZENG, Chang-jun LI, Chun-bao WANG, Quan-quan LIU, Li-hong DUAN, Tong-yang SUN, Qing SHI, Zhuo-hua LIN, Ya-jing SHEN, Wan-feng SHANG, Zheng-zhi WU
Thisÿpaperÿpresents a functional electrical stimulation system to mimic the EMG characterization of patient healthy limb to exercise his/her affected limb. Firstly, the existing problems of functional electrical stimulation system are analyzed. Generally, the existing electrical stimulation systems suffer...
Proceedings Article

Miniaturized System with a Facile Isothermal Amplification Microfluidic Chip for Rapid Detection of Zika Viruses

Xing CHEN, Lu-lu ZHANG, Da-fu CUI, Hong YIN
A real-time fluorescence detection biomedical miniaturized system with a disposable microfluidic chip was developed for loop-mediated isothermal amplification (LAMP) reactions. The miniaturized system was developed consisting of a mini heating plate, a temperature sensor, a temperature controller and...
Proceedings Article

The Development Status and Suggestions of Chinese Biological Medicine

Jin-guo WANG, Na WANG
Biological medicine is listed on the country's important development strategy planning in the United States, the European Union, Japan and other major developed countries and some developing countries. Through analysis of Chinese current situation of the development of biological medicine and compared...
Proceedings Article

The Protection of the Patients' Privacy in Anesthesiology Clinic

Na WANG, Jin-guo WANG
Patients will fall asleep under anesthesiology in most cases, so they will lose their consciousness to protect themselves. Therefore, the anesthetists carry on more duties to protect the patients' privacy.
Proceedings Article

Astaxanthin in Disease Prevention and Treatment

Astaxanthin is a carotenoid with strong antioxidant activity. The function of astaxanthin has been associated with a number of health-promoting benefits. This paper discusses the beneficial effects of astaxanthinin the prevention and treatment of human diseases such as cardiovascular diseases, cancer,...
Proceedings Article

Network Pharmacology of JinChaiKangBingDu Capsule Against Influenza A (H1N1) Virus Infection

Zhi-de CHENG, Xiao-jing WANG, Peng DENG, Jing ZHAO, Gao CHEN
Pharmacological mechanisms of Chinese herbal prescription are complicate since it contains heterogeneous components. These mechanisms are hardly understood by traditional methodology in the mass. Here we use network pharmacology approaches to explore mechanisms of JinChaiKangBingDu Capsule (JCKBD) against...
Proceedings Article

Clinical Application of Soft Ureter Mirror

Fan-Ping MENG, Yong-liang CUI, Jun WEI, Ji GAO
Objective:To summarize the clinical application of ureter soft mirror. Methods:The treatment of urinary calculi includes ESWL, percutaneous nephrolithotomy, ureter soft mirror and so on , The treatment of urinary calculi with ureter soft mirror is becoming more and more widespread, and the indications...
Proceedings Article

Research on Concanavalin A Crystallization in a Flow-Free Droplet-Based Device

Guan-ru DU, Wen-jun ZHANG, Ding-kun JI, Feng-juan CHEN, Di YIN, Hong-bo ZHANG, Rui-xue YIN, Xiao-peng HE, Shih-Mo YANG
Protein crystallography is a very critical process that limits the development of pharmaceutical engineering. Microfluidic technology has provided an opportunity for protein crystallization. In this paper, a flow-free droplet-based device is presented for producing highly controlled crystals and fabrication...
Proceedings Article

Ultrastructure and Self-Cleaning Function of Moth (Notodontidae) and Butterfly (Lycaenidae) Wings

Yan FANG, Gang SUN, Jing-shi YIN, Wan-xing WANG, Yu-qian WANG
The microstructure, hydrophobicity, adhesion and chemical composition of the butterfly and moth wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle meter, and a Fourier transform infrared spectrometer (FT-IR). Using ground calcium carbonate (heavy CaCO3) as contaminating...
Proceedings Article

A Comparison of the Special Wetting Property between Locust and Butterfly Wing

Yan FANG, Gang SUN, Jing-shi YIN, Wan-xing WANG, Yu-qian WANG
The microstructure, wettability and chemical composition of butterfly and locust wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle meter and a Fourier transform infrared spectrometer (FT-IR). The hydrophobicity models were established on the basis of Cassie equation....
Proceedings Article

Analysis of Vitiligo Model Based on Clinical Features of Traditional Chinese Medicine and Western Medicine

Xiao-li YAN, Li-hua CAO, Ming-san MIAO
Objective: Analysis common animal models and vitiligo clinical symptoms anastomotic situation, put forward recommendations of improving the model. Methods: By summarizing the characteristics and modeling methods of vitiligo model and according to the clinical diagnostic criteria of vitiligo on TCM and...
Proceedings Article

Amide Resonance Structure Detected by NMR to Predict Hydroxyl Unit in Protein

Yan JI, Xiao-liang YANG, Jun-ming TANG
Amide tautomer imidic acid were exit, which proved by NMR spectra characterization. The protein BSA was test by 1H NMR in different pH values solutions, to show the different amide/imidic acid structures exit in BSA. The amide/imidic-acid unit with H proton communication might be the biological protein...
Proceedings Article

Indirect Spectrophotometric Determination of Acetylcysteine in Pharmaceutical Sample using Fe(III)-Sulfosalicylic Acid System

Xin-rong WEN, Chang-qing TU
In acidic medium, Fe (III) can be reduced to Fe (II) by hydrosulfuryl (-SH) in acetylcysteine molecule, and then using sulfosalicylic acid as chromogenic reagent of Fe (III) to determinate the acetylcysteine indirectly by discoloration spectrophotometry.An accurate fast spectrophotometric method for...
Proceedings Article

Age Estimation Based on Skin Surface Images

Xiang-qian HE, Ling-yu LI, Zheng JIANG, Hao LI
This paper presents a model of detection age which quantifies the appearance changes of human skin at different stages of aging without traditional skeleton. We provide a novel method to extract age-related primitive features from skin surface images obtained by a digital camera, including skin surface...
Proceedings Article

Targeted Therapies for Alzheimer's Disease: An Overview

Yong SUN, Ying-ju XIU, Ying XING
Dementia is the most common onset of Alzheimer's disease (AD). The main neuropathological characteristics of AD are extracellular amyloid plaques and intracellular accumulation of hyperphosphorylated Tau protein. Because of the complexity of the pathogenesis, the most treatment options are not effective...
Proceedings Article

Use Text Mining and Complex Networks to Analyze TCM Syndromes

Xing ZHAI, Ping WANG, You-liang HUANG
Objective: To make intrinsic biological distinctions between qi deficiency pattern and qi stagnation pattern with genes related to NEI. Methods: Establish a data dictionary of NEI-related genes and a keyword lexicon of qi deficiency pattern and qi stagnation pattern. Then retrieve relevant literature...
Proceedings Article

Preliminary Study of LAMP Method for the Detection of Equine Infectious Anemia Virus

Jia-qi HAN, Han ZHANG, Qin TANG, Ting-chuan LIU, Jing XU, Li -rui LI, Gang YU, Yin WANG, Xue-ping YAO, Ze-xiao YANG
To develop a rapid molecular biological method for the detection of equine infectious anemia virus (EIAV), a set of 4 primers were designed according to the conserved sequence fragment of the gag non-structure protein gene of EIAV published in GenBank, and the reverse transcriptase loop-mediated isothermal...
Proceedings Article

New Generation Cardiac SPECT Systems

Jin-hua SHENG, Jian-hai ZHANG
The cardiac SPECT is a good study because of the large clinical demand and the need for improved image quality. SPECT imaging usually suffers from poor spatial resolution and high statistical noise due to patient radiation safety concerns. Scientists proposed some high count sensitivity cardiac SPECT...
Proceedings Article

Cerebral Microbleed Detection by Wavelet Entropy and Naive Bayes Classifier

Hai-nan WANG, Beatrice Gagnon
(Aim) Current cerebral microbleed detection methods are too complicated, and difficult to train. (Method) We enrolled 10 subjects diagnosed as cerebral microbleed.Our method combined wavelet entropy and naive Bayes classifier. (Results) The simulation results over 10 times of 10-fold cross validation...
Proceedings Article

Correction of Sleep Staging Based on Sleep Spindle Detection

Xue-mei JIN, Jun-zhong ZOU, Jian ZHANG
It has been demonstrated that sleep has a great relationship with humans' health and activities. Sleep staging is an important method to analysis sleep. Lots of methods have been adopted for automatic sleep staging. Some of the results are good and some of them are poor. For the poor, the sleep spindle...
Proceedings Article

MEG Weak Source Imaging Methods Based on Functional Connectivity

Ting WU, Yuan CUI, Zhi-guo ZHANG, Jun-peng ZHANG, Tian-zi JIANG
Beam former, as a class of Magnetoencephalography (MEG) source imaging methods, is widely used. However, it has difficulty in locating weak electromagnetic sources within brain. To solve this problem, this paper attempts to develop a method for MEG weak Source Imaging. Firstly classical beam former is...
Proceedings Article

Effects of Jinjiang Granules on Immunologic Function of Immunosuppressive Mice Model Induced by Cyclophosphamide

Ming-san MIAO, Li-hua CAO, Xue-xia ZHANG
Objective: To explore the effects of jinjiang granules on immunologic function and to provide evidence for jinjiang granules as the application of clinical medicine in the initial stage of cold, through the observation of the effects of jinjiang granules on immunologic function of low immune mouse model...
Proceedings Article

Enhancement of Viability for Staphylococcus Aureus During Freeze-Drying Using the Response Surface Methodology

Hai-li YANG, Jin-xia LI, Dong-guang XIAO, Hai-bo DU, Bo LIU, Yi LIU, Hai-rong HU
Response surface methodology (RSM) was used to optimize a protective agent for enhancing the cell viability of Staphylococcus aureus during freeze-drying. Using a previous Plackett-Burman design, it was found that sodium glutamate, trehalose, L-cysteine hydrochloride and bovine serum albumin were the...
Proceedings Article

Liver Vessel Segmentation Algorithm Based on Fuzzy Enhance and Hessian Matrix

Yue-jing QIAN
This paper proposes a liver vessel segmentation algorithm based on fuzzy enhancement and Hessian matrix. First, a method with two steps based on the theory of fuzzy enhancement is proposed to enhance the liver image. The first enhancement step is to make the local changes in the image more obvious on...
Proceedings Article

Analysis of Risk Factors for Uteroplacental Apoplexy Complicating Placental Abruption: A Systematic Review

Ning LIU, Yang LI, Yang CHEN, Yuan TIAN, Fu-ju WU
The paper explored risk factors for uteroplacental apoplexy complicating placental abruption, the condition's clinical characteristics, and maternal and fetal outcomes.62 pregnancies of placental abruption were collected from Second Hospital Jilin University during Jan. 2007-Dec. 2012.According to uteroplacental...
Proceedings Article

Study on the Key Technology of Micro-arc Oxidation of TA2 Titanium for Medical Use

Yu-lei LI, Yue-lai DAI, Jin-jun TANG, Xiao-yu JIN, Qun WANG
By micro-arc oxidation technology, the TA2 titanium metal surface modification, by scanning electron microscopy (SEM) experiments, the effects of different voltage parameters on film thickness and surface microstructure were studied. The results show that as the voltage increases and the oxidation time...
Proceedings Article

A Novel Indicator for Quantitative Assessment of Articular Cartilage Degeneration Using Ultrasound

Jing-chen ZHANG, Qiong HE, Zhi-xiu HAO, Jian-wen LUO, Li-ying XIAO
Assessment of articular cartilage degeneration induced by degenerative diseases using quantitative ultrasound method is promising but challenging. Currently, in literature, proposed methods have limited diagnostic abilities, and some quantitative ultrasound cartilage evaluation results are inconsistent...
Proceedings Article

Study on the Infrared Spectra of Superoxide Dismutase with the Different Treatment Intensity of Electric Field

Siqin-gaowa BAO, Yan-ru WANG, Zhi-chao CHEN, Zhao WEI, Zhan-xin MA
With the different treatment intensity of electric field, we analyze the influence of electric field on infrared spectra of superoxide dismutase by using infrared spectra method. The results show that, superoxide dismutase (SOD) is treated with different electric field intensity, the absorption peak...
Proceedings Article

Qualities and Aroma of Watermelon Juice during Storage at 4 øC

Yu-bin WANG, Yue MA, Xiao-yan ZHAO, Chao ZHANG
The watermelon juice was pasteurized by the ultra-high temperature treatment, and stored at 4 øC for 2 weeks. The ultra-high temperature reduced the initial microbial counts of the watermelon juice. The aroma of the watermelon juice was reduced during the storage at 4 øC for 2 weeks. The typical volatiles...
Proceedings Article

Preparation on Sustained-Release Drug of Na-MMT-Miglitol

Jie-bing ZHANG, Hui SHI, Xiu-bin REN, Guang-heng WANG
In order to sucessfully prepare Na-MMT-Miglitol composite, the adsorption effect factors of the different concentrations, temperature , pH and time for miglitol were investigated respectively. By means of the adsorption capacity changes of Na-MMT, the maximum adsorption capacity of Na-MMT as the experimental...
Proceedings Article

A Micro/Nano Structural Model for Wettability of Moth Wing Surface

Yan FANG, Gang SUN, Jing-shi YIN, Wan-xing WANG, Yu-qian WANG
The micro-morphology, wettability and chemical composition of the moth wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle (CA) meter and a Fourier transform infrared spectrometer (FT-IR). The micro-morphological models for hydrophobicity on the wing surface were...
Proceedings Article

Identification of a Bacillus Thuringiensis Cry8Ea3 to Xin-Binding Alkaline Phosphatase from Holotrichiaparallela

Wei WANG, Dan ZHAO, Wei GUO, Xiao-ping YAN, Ya-kun ZHANG, Kun-li ZHAO, Yu-jie GAO, Xiao-yun WANG
In order to further study the Insecticidal Mechanism of Bt protein against Holotricia parallela. In the study, According to the results of midgut transcriptome sequencing and RACE-PCR, the full-length hpalp gene was cloned. Sequencing analysis showed that the open reading frame of hpalp (GenBank accession...