Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Miniaturized System with a Facile Isothermal Amplification Microfluidic Chip for Rapid Detection of Zika Viruses

Authors
Xing CHEN, Lu-lu ZHANG, Da-fu CUI, Hong YIN
Corresponding Author
Xing CHEN
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.64How to use a DOI?
Keywords
Microfluidic chips, Loop-mediated is other mal amplification (LAMP), Formatting, Miniaturized System.
Abstract
A real-time fluorescence detection biomedical miniaturized system with a disposable microfluidic chip was developed for loop-mediated isothermal amplification (LAMP) reactions. The miniaturized system was developed consisting of a mini heating plate, a temperature sensor, a temperature controller and an adjustable mechanical stage. Based on experimental result, the temperature of the heating plate is evenly distributed, and the error of the temperature is less than 1.3%. The microfluidic chip was designed and fabricated by MEMS technology and thin-casting method. The volume of the LAMP reaction was changed from 25 L to 2 L. The volume of the LAMP reaction was optimized. The nucleic acid of Zika viruses was amplified and detected in real-time mode with our miniatureized system by using above microfluidic chip. From the experiment results, the detection range was from 100 copies to 106 copies. This biomedical system has the potential for point-of-care diagnostics (POCT) with the many advantages, such as not high cost, short analysis and detection time, not much reagent and sample consumption and so on.
Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)

Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.64How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Xing CHEN
AU  - Lu-lu ZHANG
AU  - Da-fu CUI
AU  - Hong YIN
PY  - 2017/05
DA  - 2017/05
TI  - Miniaturized System with a Facile Isothermal Amplification Microfluidic Chip for Rapid Detection of Zika Viruses
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.64
DO  - https://doi.org/10.2991/bbe-17.2017.64
ID  - CHEN2017/05
ER  -