Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Preliminary Study of LAMP Method for the Detection of Equine Infectious Anemia Virus

Authors
Jia-qi HAN, Han ZHANG, Qin TANG, Ting-chuan LIU, Jing XU, Li -rui LI, Gang YU, Yin WANG, Xue-ping YAO, Ze-xiao YANG
Corresponding Author
Jia-qi HAN
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.79How to use a DOI?
Keywords
Equine infectious anemia, LAMP, Detection.
Abstract
To develop a rapid molecular biological method for the detection of equine infectious anemia virus (EIAV), a set of 4 primers were designed according to the conserved sequence fragment of the gag non-structure protein gene of EIAV published in GenBank, and the reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was established through identification of recombinant plasmid pMD-19T-gag which including target gene fragments, optimization of reaction conditions, sensitivity and specificity tests, and the validity test. The results showed the EIAV RT-LAMP detection method had a ladder-like pattern of amplification bands incubation at 63 for 2 hours by using agarose gel electrophoresis, the sensitivity of the RT-LAMP could reach about 100 copies/ L of target fragments, there was no amplification for Proteus, Actinobacillus, Salmonella, Escherichia coli, Stenotrophomonasmaltophilia and Streptococcus type II from equine detection by this approach. The RT-LAMP assay established here had good specificity and sensitivity, which is suitable for EIAV rapid detection.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.79How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Jia-qi HAN
AU  - Han ZHANG
AU  - Qin TANG
AU  - Ting-chuan LIU
AU  - Jing XU
AU  - Li -rui LI
AU  - Gang YU
AU  - Yin WANG
AU  - Xue-ping YAO
AU  - Ze-xiao YANG
PY  - 2017/05
DA  - 2017/05
TI  - Preliminary Study of LAMP Method for the Detection of Equine Infectious Anemia Virus
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.79
DO  - https://doi.org/10.2991/bbe-17.2017.79
ID  - HAN2017/05
ER  -