Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

A Food-Grade, Secretory Expression of IL1RA Protein in a Lactococcus Lactis System for Potential Oral Therapy on IBD

Authors
Jin CHENG, Jin CAO, Jia CAO, Xiao-feng WANG, Yan-ru GE
Corresponding Author
Jin CHENG
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.61How to use a DOI?
Keywords
Interleukin 1 receptor antagonist (IL1RA), Lactococcus lactis, Recombinant, Inflammatory bowel disease (IBD).
Abstract
Interleukin 1 (IL1 ) is a potential target for IBD treatment. IL1 receptor antagonist (IL1RA) can blocks the biologic activity of IL1 by competitively inhibiting IL1 binding to the IL1R, so IL1RA is suggested to have possible therapeutic applications in treatment of IBD. By using a food-grade recombinant Lactococcus lactis system, a constitutive expression vector IL1RA:pNZ8149 was constructed and transferred into a L. lactis host NZ3900 in this study. The engineered strain was fermented and detected by colonies PCR and Western blot, the results indicate that the IL1RA secretory expression can be archived by IL1RA/NZ3900.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.61How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Jin CHENG
AU  - Jin CAO
AU  - Jia CAO
AU  - Xiao-feng WANG
AU  - Yan-ru GE
PY  - 2017/05
DA  - 2017/05
TI  - A Food-Grade, Secretory Expression of IL1RA Protein in a Lactococcus Lactis System for Potential Oral Therapy on IBD
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.61
DO  - https://doi.org/10.2991/bbe-17.2017.61
ID  - CHENG2017/05
ER  -