Enhancement of Viability for Staphylococcus Aureus During Freeze-Drying Using the Response Surface Methodology

DOI

10.2991/bbe-17.2017.85How to use a DOI?

Keywords

Freeze-drying, Protective agents, Response surface methodology, Staphylococcus aureus, Storage.

Abstract

Response surface methodology (RSM) was used to optimize a protective agent for enhancing the cell viability of Staphylococcus aureus during freeze-drying. Using a previous Plackett-Burman design, it was found that sodium glutamate, trehalose, L-cysteine hydrochloride and bovine serum albumin were the most effective freeze-drying protective agents for S. aureus. Through the comparative analysis of single protectant, the complex protective agents show better effect on the S. aureus viability. The paper was carried out by Box-Behnken Design to determine the optimum levels of these four protective agents to seek for maximum viability of S. aureus during freeze-drying. The experimental data allowed the development of an empirical model (P<0.0001) describing the inter relationships between the independent and dependent variables. By solving the regression equation, and analyzing the response surface contour and surface plots, the optimal concentrations of the agents were determined as: 15.73% sodium glutamate, 3.43% trehalose, 0.50% L-cysteine hydrochloride, 0.47% bovine serum albumin. S. aureus freeze dried in this medium obtained a cell viability of up to 96.78%.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hai-li YANG
AU  - Jin-xia LI
AU  - Dong-guang XIAO
AU  - Hai-bo DU
AU  - Bo LIU
AU  - Yi LIU
AU  - Hai-rong HU
PY  - 2017/05
DA  - 2017/05
TI  - Enhancement of Viability for Staphylococcus Aureus During Freeze-Drying Using the Response Surface Methodology
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 531
EP  - 539
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.85
DO  - 10.2991/bbe-17.2017.85
ID  - YANG2017/05
ER  -