Enhancement of Viability for Staphylococcus Aureus During Freeze-Drying Using the Response Surface Methodology
- Hai-li YANG, Jin-xia LI, Dong-guang XIAO, Hai-bo DU, Bo LIU, Yi LIU, Hai-rong HU
- Corresponding Author
- Hai-li YANG
Available Online May 2017.
- https://doi.org/10.2991/bbe-17.2017.85How to use a DOI?
- Freeze-drying, Protective agents, Response surface methodology, Staphylococcus aureus, Storage.
- Response surface methodology (RSM) was used to optimize a protective agent for enhancing the cell viability of Staphylococcus aureus during freeze-drying. Using a previous Plackett-Burman design, it was found that sodium glutamate, trehalose, L-cysteine hydrochloride and bovine serum albumin were the most effective freeze-drying protective agents for S. aureus. Through the comparative analysis of single protectant, the complex protective agents show better effect on the S. aureus viability. The paper was carried out by Box-Behnken Design to determine the optimum levels of these four protective agents to seek for maximum viability of S. aureus during freeze-drying. The experimental data allowed the development of an empirical model (P<0.0001) describing the inter relationships between the independent and dependent variables. By solving the regression equation, and analyzing the response surface contour and surface plots, the optimal concentrations of the agents were determined as: 15.73% sodium glutamate, 3.43% trehalose, 0.50% L-cysteine hydrochloride, 0.47% bovine serum albumin. S. aureus freeze dried in this medium obtained a cell viability of up to 96.78%.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Hai-li YANG AU - Jin-xia LI AU - Dong-guang XIAO AU - Hai-bo DU AU - Bo LIU AU - Yi LIU AU - Hai-rong HU PY - 2017/05 DA - 2017/05 TI - Enhancement of Viability for Staphylococcus Aureus During Freeze-Drying Using the Response Surface Methodology BT - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017) PB - Atlantis Press SN - 2468-5747 UR - https://doi.org/10.2991/bbe-17.2017.85 DO - https://doi.org/10.2991/bbe-17.2017.85 ID - YANG2017/05 ER -