Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Identification of a Bacillus Thuringiensis Cry8Ea3 to Xin-Binding Alkaline Phosphatase from Holotrichiaparallela

Authors
Wei WANG, Dan ZHAO, Wei GUO, Xiao-ping YAN, Ya-kun ZHANG, Kun-li ZHAO, Yu-jie GAO, Xiao-yun WANG
Corresponding Author
Wei WANG
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.94How to use a DOI?
Keywords
Holotriciaparallela, Alkaline phosphatase, Ligand blot, qRT-PCR.
Abstract
In order to further study the Insecticidal Mechanism of Bt protein against Holotricia parallela. In the study, According to the results of midgut transcriptome sequencing and RACE-PCR, the full-length hpalp gene was cloned. Sequencing analysis showed that the open reading frame of hpalp (GenBank accession NO. KY922835) was 1605 bp long, encoding 534 amino acid residues. The predicted molecular weight and isoelectric point of HpALP were 59 kDa and 5.18, respectively. Analysis of HpALP signal peptide with 21 amino acids, GPI anchor point is located in the C-terminal D514, and has two N-glycosylation sites: N100, N296. The results of Ligand blot showed that the binding of HpALP with Cry8Ea3 toxin. Transcriptional analysis of hpalp in different tissues of H. parallela larvae was performed by qRT-PCR, which revealed that the hpalp was primarly expressed higher in midgut, but lower in the foregut.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.94How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Wei WANG
AU  - Dan ZHAO
AU  - Wei GUO
AU  - Xiao-ping YAN
AU  - Ya-kun ZHANG
AU  - Kun-li ZHAO
AU  - Yu-jie GAO
AU  - Xiao-yun WANG
PY  - 2017/05
DA  - 2017/05
TI  - Identification of a Bacillus Thuringiensis Cry8Ea3 to Xin-Binding Alkaline Phosphatase from Holotrichiaparallela
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.94
DO  - https://doi.org/10.2991/bbe-17.2017.94
ID  - WANG2017/05
ER  -