Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Extracellular Expression and Catalytic Bioactivity of Optimized Human Aromatase (CYP19) in Pichia Pastoris

Authors
Yan-ying HU, Yong-hui ZHONG, Jing ZHEN, Hui-tu ZHANG, Shi-ru JIA, Fu-ping LU, Yu-jie DAI, Xiu-li ZHANG
Corresponding Author
Yan-ying HU
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.3How to use a DOI?
Keywords
Recombinant aromatase, Pichia pastoris, Heterologous protein production.
Abstract
The fragment encoding aromatase (CYP19) with 45 N-terminal amino acids cut-off was cloned into pPIC9K vector and transformed to the yeast Pichia pastoris. The multi-copy recombinant strain was selected for the production of recombinant aromatase. The catalytic bioactivity of secreted enzyme was further verified by fluorescent substrate detection method. The results indicate that aromatase was successfully expressed in recombinant Pichia pastoris strain and secreted into the culture supernatant. The extracellular expression of the bioactive aromatase by P. pastoris provides us a better alternative for aromatase commercial production and promotes the research progress for the discovery of novel aromatase.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.3How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yan-ying HU
AU  - Yong-hui ZHONG
AU  - Jing ZHEN
AU  - Hui-tu ZHANG
AU  - Shi-ru JIA
AU  - Fu-ping LU
AU  - Yu-jie DAI
AU  - Xiu-li ZHANG
PY  - 2017/05
DA  - 2017/05
TI  - Extracellular Expression and Catalytic Bioactivity of Optimized Human Aromatase (CYP19) in Pichia Pastoris
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.3
DO  - https://doi.org/10.2991/bbe-17.2017.3
ID  - HU2017/05
ER  -