Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Fed-Batch Fermentation of L- Threonine by Escherichia Coli Supplemented with B-Vitamins

Authors
Yue-wen SU, Qun-qun GUO, Sen WANG, Xin ZHANG, Jian WANG
Corresponding Author
Yue-wen SU
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.47How to use a DOI?
Keywords
Escherichia coli, L-threonine, Fed-batch fermentation, B-vitamins.
Abstract
B-vitamins can be used as cofactors in the synthesis process of L-threonine. Concentration of the cofactor can effectively change the activity of specific enzymes and reallocate the distribution of carbon substances in the cells. Theeffects of B-vitamins, including calcium pantothenate (VB5), cobalamin (VB12), choline chloride (VB4) and nicotinamide (VB3), on L-threoninefermentation by fed-batch culture of Escherichiacoli JLTHRin a 5L fermentorwere investigated. The results showed that the production of L-threonine was increased to 133.4 g/L, which was increased by 7.8% when supplemented with 1 g/L VB4.The production of L-threonine was increased to 130.6 g/L, which was increased by 5.6%when supplemented with10mg/L VB3. At last, when a combination of 1g/L VB4 and 10mg/L VB3 was added to the glucose solutionwith1.5g/L betainehydrochloride, 138.4g/LL-threonine was accumulated, which was increased by 11.9%.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.47How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yue-wen SU
AU  - Qun-qun GUO
AU  - Sen WANG
AU  - Xin ZHANG
AU  - Jian WANG
PY  - 2017/05
DA  - 2017/05
TI  - Fed-Batch Fermentation of L- Threonine by Escherichia Coli Supplemented with B-Vitamins
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.47
DO  - https://doi.org/10.2991/bbe-17.2017.47
ID  - SU2017/05
ER  -