Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Aqueous Two-Phase System Combined with Ultrasound for the Extraction Of Polysaccharide from Hawthorn (Crataeguspinnatifida) Leaves

Authors
Ri-li HAO, Juan-juan MA, Kun LI, Chuan-he ZHU, Xiao-cun ZHANG
Corresponding Author
Ri-li HAO
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.37How to use a DOI?
Keywords
Hawthorn Leaves, Polysaccharide, Aqueous Two-Phase System, Ultrasound, Response Surfacemethodology.
Abstract
Aqueous two-phase system (ATPS) combined with ultrasonic technology was employed to extract polysaccharide from hawthorn leaves in this study. The partition behaviors in different ATPS were investigated first, polyethylene glycol (PEG) 11.5% and (NH4)2SO4 14% were selected as the optimum solvent for extracting polysaccharide. Using the optimum ATPS solvent, the ratio of material to solvent, The time, temperature and power of the ultrasonic treatment were investigated using a single factor experiment and Box-Behnken Design (BBD) of response surface methodology (RSM) to obtain the optimum conditions for extracting polysaccharide. The optimum conditions were the ratio of material to solvent 1:34, ultrasonic time (UT) 35 min, ultrasonic power (UP) 300 w and ultrasonic temperature (UTE) 65 . With the optimal conditions, the maximum yield of polysaccharide was 3.05%. The present study provided a foundation for further purification of polysaccharide and offered a valuable alternative for extracting polysaccharide from plants.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.37How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Ri-li HAO
AU  - Juan-juan MA
AU  - Kun LI
AU  - Chuan-he ZHU
AU  - Xiao-cun ZHANG
PY  - 2017/05
DA  - 2017/05
TI  - Aqueous Two-Phase System Combined with Ultrasound for the Extraction Of Polysaccharide from Hawthorn (Crataeguspinnatifida) Leaves
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.37
DO  - https://doi.org/10.2991/bbe-17.2017.37
ID  - HAO2017/05
ER  -