Time-Dependent Cellular Injuries Induced by Calcium Oxalate Monohydrate and Dihydrate Crystals

DOI

10.2991/bbe-17.2017.5How to use a DOI?

Keywords

Calcium oxalate, time effect, cellular injury, renal epithelial cells.

Abstract

Objective: This study aims to compare the cytotoxicity and adhesion difference between oxalate monohydrate (COM) and dehydrate (COD) crystals and African green monkey kidney epithelial cells (Vero). Methods: Through the detection of cell viability, lactate dehydrogenase (LDH) release amount, expression of hyaluronic acid, and propidium iodide staining method to compare the cell injury degree; scanning electron microscope was used to observe crystal adhesion. Results: Cellular damage increased as time extended within 1~12 h. The cytotoxicity of COM was stronger than COD, and the adhesion amount of COM was much larger than COD. Conclusions: The formation of large amount of micron-sized COM crystals during short time in urine will produce cytotoxicity to kidney epithelial cells, which increasing the risk of stone formation, In contrast, the formation of COD cause relatively less cytotoxicity.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jian-Min WANG
AU  - Ling-Hong HUANG
AU  - Jian-Ming OUYANG
PY  - 2017/05
DA  - 2017/05
TI  - Time-Dependent Cellular Injuries Induced by Calcium Oxalate Monohydrate and Dihydrate Crystals
BT  - Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SP  - 25
EP  - 31
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.5
DO  - 10.2991/bbe-17.2017.5
ID  - WANG2017/05
ER  -