Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Time-Dependent Cellular Injuries Induced by Calcium Oxalate Monohydrate and Dihydrate Crystals

Authors
Jian-Min WANG, Ling-Hong HUANG, Jian-Ming OUYANG
Corresponding Author
Jian-Min WANG
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.5How to use a DOI?
Keywords
Calcium oxalate, time effect, cellular injury, renal epithelial cells.
Abstract
Objective: This study aims to compare the cytotoxicity and adhesion difference between oxalate monohydrate (COM) and dehydrate (COD) crystals and African green monkey kidney epithelial cells (Vero). Methods: Through the detection of cell viability, lactate dehydrogenase (LDH) release amount, expression of hyaluronic acid, and propidium iodide staining method to compare the cell injury degree; scanning electron microscope was used to observe crystal adhesion. Results: Cellular damage increased as time extended within 1~12 h. The cytotoxicity of COM was stronger than COD, and the adhesion amount of COM was much larger than COD. Conclusions: The formation of large amount of micron-sized COM crystals during short time in urine will produce cytotoxicity to kidney epithelial cells, which increasing the risk of stone formation, In contrast, the formation of COD cause relatively less cytotoxicity.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.5How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Jian-Min WANG
AU  - Ling-Hong HUANG
AU  - Jian-Ming OUYANG
PY  - 2017/05
DA  - 2017/05
TI  - Time-Dependent Cellular Injuries Induced by Calcium Oxalate Monohydrate and Dihydrate Crystals
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.5
DO  - https://doi.org/10.2991/bbe-17.2017.5
ID  - WANG2017/05
ER  -