Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Injury Difference of the Un-Aggregated and Aggregated Calcium Oxalate Monohydrate on Human Kidney Proximal Tubular Epithelial Cells

Authors
Li-shan HUANG, Da GUO, Jian-ming OUYANG
Corresponding Author
Li-shan HUANG
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.26How to use a DOI?
Keywords
Cell Modulation, Calcium Oxalate, Crystal Aggregation, Osteopontin.
Abstract
Purpose: To study the injury difference of the un-aggregated and aggregated calcium oxalate monohydrate (COM) on human kidney proximal tubular epithelial (HKC) cells, and to discuss the molecular and cellular mechanism of kidney stone formation. Methods: The cell injure by crystals was evaluated by measuring the cell viability, malonaldehyde (MDA) release, and the expression level of osteopontin (OPN). Scanning electron microscope (SEM) was used to observe the HKC-COM crystal adhesion. Results: After adhesion to COM, cell vitality dropped, both MDA release and OPN expression increased. The crystal injury in HKC was in a time-dependent manner within 24 h. aggregated COM caused more serious injury than un-aggregated COM, and the adhesive capacity of aggregated COM was also obviously stronger than un-aggregated COM. Conclusions: Aggregated COM crystals enhanced the risk of calcium oxalate stone formation much stronger than un-aggregated COM crystals.
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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.26How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Li-shan HUANG
AU  - Da GUO
AU  - Jian-ming OUYANG
PY  - 2017/05
DA  - 2017/05
TI  - Injury Difference of the Un-Aggregated and Aggregated Calcium Oxalate Monohydrate on Human Kidney Proximal Tubular Epithelial Cells
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.26
DO  - https://doi.org/10.2991/bbe-17.2017.26
ID  - HUANG2017/05
ER  -