Injured Human Kidney Proximal Tubular Epithelial Cells Modulate Nucleation and Growth of Calcium Oxalate Crystals
Da GUO, Jian-min WANG, Jian-ming OUYANG
Available Online May 2017.
- https://doi.org/10.2991/bbe-17.2017.27How to use a DOI?
- HKC cells, H2O2, Injury, Calcium oxalate, Nucleation, Growth.
- Purpose: The oxidative damage of hydrogen peroxide (H2O2) on human kidney proximal tubular epithelial cell (HKC) and the nucleation and growth of calcium oxalate (CaOx) crystals modulated by normal and injured cells were investigated. Methods: The method of CCK-8 was used to detect the proliferating inhibition effect of H2O2 on HKC. Cells were incubated with serum-free culture media containing CaOx supersaturated solutions. The cellular morphology and crystal growth were observed by invert microscope and scanning electron microscope (SEM). Results: H2O2 could damage HKC in time-dependent (0~60 min) and concentration-dependent manners (0~1.0 mmol/L). The amount of CaOx crystals induced by injured cells was more than that induced by control cells, and the size of the crystals induced by injured cells was also larger. An oxidative damage mode for H2O2 was established. Conclusions: HKC could be damaged obviously by both H2O2 and high concentration CaOx solution in time-dependent and concentration-dependent manners. Injured cells promote the nucleation and growth of CaOx crystals.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Da GUO AU - Jian-min WANG AU - Jian-ming OUYANG PY - 2017/05 DA - 2017/05 TI - Injured Human Kidney Proximal Tubular Epithelial Cells Modulate Nucleation and Growth of Calcium Oxalate Crystals PB - Atlantis Press SP - 165 EP - 170 SN - 2468-5747 UR - https://doi.org/10.2991/bbe-17.2017.27 DO - https://doi.org/10.2991/bbe-17.2017.27 ID - GUO2017/05 ER -