Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye

Authors
Pin-san XU, Zheng-yao ZHANG, Ling-yao LI, Yu-bo LIU
Corresponding Author
Pin-san XU
Available Online May 2017.
DOI
https://doi.org/10.2991/bbe-17.2017.18How to use a DOI?
Keywords
Brassica juncea, Mercuric ion, Fluorescent dye.
Abstract
Concern over mercury cytotoxicity inside live specimens has encouraged the development of efficient methods for the in vivo detection of this heavy metal. In this study, by using the previous developed fluorescence dye EPNP (2,6-bis-(4'-peperazino-N'-butyl-1',8'-naphthalimide) dimethylpyridine) as a highly selective and sensitive probe for mercuric ions (Hg2+), the images of Hg2+ distribution and transportation in the living cells and plants of Brassica juncea were obtained. After 10, 50, 100 M Hg2+ exposure, gradually increased fluorescence was detected in B. juncea living cells loaded with EPNP dye. Intracellular Hg2+ time-dependent fluorescence enhancement of EPNP was also observed in B. juncea cells. Furthermore, images of confocal microscope shown that Hg2+ was found to mostly accumulate in lytic vacuole but not in the nucleus or mitochondria. In addition, a concentration-dependent rise of fluorescence was observed when the EPNP interacted with an increasing concentration of Hg2+ in the plants of B. juncea. These results provided direct experimental evidences for the distribution, translocation and transportation of Hg2+ in B. juncea. EPNP would be used as a chemical tool for the study of phytoremediation related researches.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
Part of series
Advances in Biological Sciences Research
Publication Date
May 2017
ISBN
978-94-6252-362-3
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-17.2017.18How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Pin-san XU
AU  - Zheng-yao ZHANG
AU  - Ling-yao LI
AU  - Yu-bo LIU
PY  - 2017/05
DA  - 2017/05
TI  - Visible Study of Mercuric Ion in Brassica Juncea Living Cells and Plants Using a Fluorescent Dye
BT  - 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-17.2017.18
DO  - https://doi.org/10.2991/bbe-17.2017.18
ID  - XU2017/05
ER  -