Green Synthesis of Silver Nanoparticles from Madhuca longifolia and Its Antibiofilm Potential

DOI

10.2991/978-94-6463-020-6_16How to use a DOI?

Keywords

Madhuca longifolia; Silver nanoparticles; TEM; SEM; XRD; FTIR

Abstract

The synthesis of silver nanoparticles using plant extract as a capping agent has been very easy, economical and environment friendly method. The Madhuca longifolia is one of the well-known trees for its various benefits from food industry to its medicinal applications. It is indigenous to India, Nepal, Sri Lanka and Myanmar. In the present aqueous extract was used from leaves of M. lngifolia has been used as a capping agent to form AgNPs by reducingsilver nitrate with the help of green synthesis route. UV-visible spectroscopicy gave maximaat 420 nmconfirmed the synthesis of M. longifolia AgNPs. Characterization was done by TEM, SEM, XRD and FTIR techniques. FTIR confirmed the presence of various phytochemicals and formation of nanoparticles. XRD confirmed the formation of crystalline structure of synthesized silver nanoparticles. The shape of silver nanoparticles was irregular and spherical. The reaction solution turned brown which is the primary indication of formation of AgNPs. Crystalline size was calculated and found to be 10–25 nm; and TEM showed the size of nanoparticles around 5–40 nm. The M. longifolia based Silver nanoparticles were evaluated for their antibacterial and antibiofilm activity on Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) by disc diffusion and percentage inhibition methods was confirmed.

Copyright

© 2023 The Author(s)

Open Access

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TY  - CONF
AU  - Poonam Kaithal
AU  - Rajiv Kant
AU  - Rohit Lall
AU  - Archana Verma
AU  - Preetam Verma
PY  - 2022
DA  - 2022/12/14
TI  - Green Synthesis of Silver Nanoparticles from Madhuca longifolia and Its Antibiofilm Potential
BT  - Proceedings of the conference BioSangam 2022: Emerging trends in Biotechnology (BIOSANGAM 2022)
PB  - Atlantis Press
SP  - 156
EP  - 167
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-020-6_16
DO  - 10.2991/978-94-6463-020-6_16
ID  - Kaithal2022
ER  -