RAPD-PCR-Based Genetic Relationships Among Three Forensically Important Calliphorid Species (Diptera: Calliphoridae)

DOI

10.2991/978-94-6463-020-6_24How to use a DOI?

Keywords

RAPD-PCR; Calliphoridae; Genetic relationship; Molecular markers; TFPGA

Abstract

Molecular genetic markers have been effectively used to analyze genetic relationships and diversity among different groups of dipterans. The emergence of Polymerase Chain Reaction (PCR) facilitated analysis of molecular markers e.g., Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR), has contributed a great deal in resolving the genetic relatedness in many dipterans of medical, veterinary, and economic importance. In the present study, an attempt has been made to explore the phylogenetic relationships among three calliphorid species, namely Hemipyrellia pulchra (Weidemann) and Lucilia cuprina (Weidemann), Chrysomya megacephala (Fabricius), employing Random Amplified Polymorphic DNA - Polymerase Chain Reaction (RAPD-PCR) technique using twenty random decamer primers. Complete genomic DNA was isolated from the three species and amplified by PCR using twenty random decamer primers. A total of 285 bands ranging from 141 bp to 2648 bp were generated. Tools for population genetic analysis (TFPGA) software was used to calculate genetic identity among the three species. A close relationship among the three species is reflected by high values of mean Genetic identity (0.661–0.713).

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Shubhra Malviya
AU  - Sarita Agrawal
PY  - 2022
DA  - 2022/12/14
TI  - RAPD-PCR-Based Genetic Relationships Among Three Forensically Important Calliphorid Species (Diptera: Calliphoridae)
BT  - Proceedings of the conference BioSangam 2022: Emerging trends in Biotechnology (BIOSANGAM 2022)
PB  - Atlantis Press
SP  - 247
EP  - 254
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-020-6_24
DO  - 10.2991/978-94-6463-020-6_24
ID  - Malviya2022
ER  -