Proceedings of the 2015 International Forum on Bioinformatics and Medical Engineering

The Study on Changes of NKT Cells Levels in Peripheral Blood of Children with Asthma

Authors
Huilan Zhu, Xinxin Xin, Hongyan Zhang
Corresponding Author
Huilan Zhu
Available Online September 2015.
DOI
10.2991/bme-15.2015.26How to use a DOI?
Keywords
Asthma; Nature killer cell; T cell
Abstract

Abstract. Objective: To study the changes of natural killer T cells (NKT cells)in peripheral blood and the ability of NKT cells to proliferate in response. Methods: 80 bronchial asthma children and 40 healthy children were included. NKT cells and T cells were analyzed by flow cytometry instrument. The levels of IL-4, IFN- , IL-10 were tested in different period of NKT cell culture. Results: There were 46 cases were acute, 34 cases were remission. Between the three groups, CD4+, CD8+, CD4+/CD8+ and NKT cells percentage comparison difference were statistically significant (P<0.05). CD4 +, CD4 + / CD8 + percentage in acute episodes were higher than the remission group and normal group, but CD8 +, NKT cells percentage were lower than the remission group and normal group. The difference was statistically significant (P < 0.05). There were no statistically significant difference he remission group between remission group and normal group (P > 0.05). With the extension of incubation time, IL – 4 levels in three groups of children were significantly increased, and acute attack group were higher than that in remission group and normal group. The difference was statistically significant (P < 0.05). Serums IL - 10 in cultivating 48 h raised highest, cultivate 72h declined, and acute attack group were higher than that in remission group and normal group. The difference was statistically significant (P < 0.05). Conclusion: The pathogenesis of asthma may be related to the ratio and dysfunction of NKT cells.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
Proceedings of the 2015 International Forum on Bioinformatics and Medical Engineering
Series
Advances in Engineering Research
Publication Date
September 2015
ISBN
10.2991/bme-15.2015.26
ISSN
2352-5401
DOI
10.2991/bme-15.2015.26How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Huilan Zhu
AU  - Xinxin Xin
AU  - Hongyan Zhang
PY  - 2015/09
DA  - 2015/09
TI  - The Study on Changes of NKT Cells Levels in Peripheral Blood of Children with Asthma
BT  - Proceedings of the 2015 International Forum on Bioinformatics and Medical Engineering
PB  - Atlantis Press
SP  - 143
EP  - 147
SN  - 2352-5401
UR  - https://doi.org/10.2991/bme-15.2015.26
DO  - 10.2991/bme-15.2015.26
ID  - Zhu2015/09
ER  -