Proceedings of the 2016 International Conference on Biological Sciences and Technology

The Research on the Mechanism of Antioxidative and Growth-Promoting Effects of Polyphenols from the Involucres of Castanea Mollissima Blume on IEC-6 Cells

Authors
Y. Xiong, X. Zhao, S. Dong, K.J. Guo
Corresponding Author
Y. Xiong
Available Online January 2016.
DOI
10.2991/bst-16.2016.15How to use a DOI?
Keywords
IEC-6 cells, Polyphenolic extract from involucres of Castanea mollissima Blume, RT-PCR, Western blotting.
Abstract

The aim of the paper was to study the effects of Polyphenolic extract from involucres of Castanea mollissima Blume (PICB) on IEC-6 cell proliferation and growth and antioxidant status. The IEC-6 heat stress model was established and IEC-6 cells of control group and heat stress group were treated with 80µg/ml PICB. HSP70 ODC EGF, EGFR, SOD mRNA transcription and protein expression of IEC-6 cells were detected using RT-PCR and Western blot. The results showed that PICB concentrations had no significant effect (P > 0.05) on mRNA and protein expression of IEC-6 in normal temperature. Under heat stress conditions, HSP70, ODC, EGF, EGFR, SOD mRNA transcription and protein expression of PICB group were higher than the control group (P<0.05). In conclusion, PICB could up-regulate HSP70, ODC, EGF, EGFR, SOD mRNA and protein expression of heat stressed IEC-6 cell, showing the mechanism of its anti-oxidative and growth-promoting function.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Conference on Biological Sciences and Technology
Series
Advances in Biological Sciences Research
Publication Date
January 2016
ISBN
10.2991/bst-16.2016.15
ISSN
2468-5747
DOI
10.2991/bst-16.2016.15How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Y. Xiong
AU  - X. Zhao
AU  - S. Dong
AU  - K.J. Guo
PY  - 2016/01
DA  - 2016/01
TI  - The Research on the Mechanism of Antioxidative and Growth-Promoting Effects of Polyphenols from the Involucres of Castanea Mollissima Blume on IEC-6 Cells
BT  - Proceedings of the 2016 International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SP  - 92
EP  - 97
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.15
DO  - 10.2991/bst-16.2016.15
ID  - Xiong2016/01
ER  -