Proceedings of the 2016 International Conference on Biological Sciences and Technology

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53 articles

Clinical Practicability of Extending the Length of Gastrointestinal Decompression Cathetering

Lei Sun, Xin-Quan Gu, An-Na Wang, Fan-Ping Meng
Objective: We intended to investigate the appropriate length of the stomach tube in the treatment of gastrointestinal decompression. Material and Methods: The patients in treatment of gastrointestinal decompression cured by our department from January 2013 to May 2015 whom were divided into 2 groups...

Quantitative Structure-Toxicity Relationship for Predicting Acute Toxicity of Phenols

Zhi-Xiang Zhou, Meng-Nan Qin, Yang-Hua Liu, Xiao-Long Zhang, Han-Dong Li
Phenols represent one of the most important classes of environmental chemicals. Most of them may cause serious public health and environmental problems. The present work is to develop an effective QSTR model for acute toxicity, a toxicological endpoint of Phenols. We calculated various descriptors and...

Research on Morphology of Human Red Blood Cells (RBCs) in Different Gravity Fields

Lin Li, Shuang-Yin Gao
By testing fresh human red blood cells (RBCs), the morphology of human RBCs in different gravity fields was observed by SK2109P microscope. And the death rate of RBCs in different gravity fields were counted Results show that: RBCs can endure a certain gravity field. RBCs barely died under gravity fields...

To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus

Ying-Ying Liu, Jing Wang
Acridine orange (AO) and ethidium bromide (EB) are two fluorescent dyes for cell nuclei staining. The nucleus images by these two staining can make it easy to differentiate white blood cell (WBC) from red blood cell (RBC). We applied this principle to the white blood cell count by hemocytometer and this...

Transurethral Flexible Ureteroscopic Holmium Laser Lithotripsy for Treatment of Kidney and Upper Ureteral Calculi

Fan-Ping Meng, An-Na Wang, Ji Gao
This paper aims at studying the efficacy and safety of flexible ureteroscopic holmium laser lithotripsy for treatment of kidney and upper ureteral calculi. Methods. The duration of operation, postoperative complication, postoperative calculus clearance rate of this surgery were analyzed based on 58 patients...

Investigation of Listeria Phage Endolysin Cell-wall Binding Domain (CBD) Surface Display in Escherichia coli

Shan-Na Liu, Timo M. Takala, Justus Reunanen, Ossian Saris, Per E. J. Saris
Cell surface display of target proteins has been widely used in biotechnology and industry. In this study, cell-wall binding domain (CBD) from Listeria phage endolysin A500 was fused with anchoring domains of YadA and OmpA, respectively, in Escherichia coli, aiming at binding of E. coli cells to Listeria...

Effect of Carnosine on Physico-Chemical and Antioxidant Activity Properties of FSG-CaCO3 Composite Films

Li-Hui Wang, Le Zhang, An-Jun Liu
For the sake of developing fish skin gelatin and CaCO3 composite films with antioxidant activity, different concentration of carnosine was added to it. In addition, the mechanical properties, optical properties, surface topography, anti-microbial activity, antioxidant activity and preservation effect...

Gene Prediction Based On a Generalized Hidden Markov Model and Some Statistical Models of Related States: a Review

Rui Guo, Jian Zhang, Ke Yan, Tian-Qi Wang
In recent years, the methods with a generalized hidden Markov model have gained significant application and development in gene prediction, which is predicting the location and structure of genes in genomic sequences, and produced an army of remarkable programs, such as Genie, GENSCAN, AUGUSTUS, etc....

The Separation and Purification of Se-enriched Mycelium Polysaccharides of Catathelasma Ventricosum and Their Anti-hyperglycemic and Antioxidant Activity

Yun-Tao Liu, Qian-Qian Tang, Yu-Xian You, Si-Qi Zeng, Yi-Wen Li, Di Chen, Ai-Ping Liu, Chao-Hui Feng, Zhao Su, Cheng Li, Dai-Wen Chen
Selenium (Se) is an essential nutrient for humans as it plays an important role in anti-hyperglycemic and antioxidant activities. The objective of this work was to evaluate anti-hyperglycemic and antioxidant activities of Se-enriched mycelium polysaccharides. The Se-enriched mycelium polysaccharides...

Differences between Aureobasidium Pullulans CGMCC No.3337 and Aureobasidium Pullulans TKPM10017 on Synthesis of Poly (β-L-malic-acid) Metabolism

Hai-Song Yin, Yan-Hui Bian, Wei-Hua Tang, Xu-Jia Fan, Zheng Li, Chang-Sheng Qiao
Poly (β-L-malic-acid) (PMLA) is a sort of polyester and absolute biodegradable material. PMLA is a new type of nontoxic macromolecule material and with favorable biodegradation and biocompatibility. PMLA has extensive applications in the areas of pharmacy and medical industry. Application foreground...

Preparation of Diuridine and Dicytidine Pentaphosphates

Yue-Hai You, Shan-Shan Gong, Qi Sun
Two symmetrical dinucleoside pentaphosphates, Up5U and Cp5C, were synthesized via the activation of the P (V)–N bond. The key nucleoside phosphoropiperidates intermediate were obtained by a redox condensation method. The reaction of the phosphoropiperidate with uridine and cytidine tetraphosphate in...

Immunoassays of Methionine Adenosyltransferase Activity, S-adenosylmethione and Their Applications

Xiu-Juan Hao, Hui-Jun Li, Min Zhou
To understand how S-adenosylmethione (SAM) fluctuates in normal and cancerous liver cells, we developed novel methods for measuring methionine adenosyltransferase (MAT) activity and SAM. This method integrated MAT-catalyzed reaction and immunoassay that specifically and quantifies product SAM. IHC, IF...

Coagulation Factor XII –A Key Pro-Inflammatory and Pro-Coagulant Protein

Shan-Shan Wang, Jin-Wen Ge, Shao-Wu Cheng
Coagulation factor XII (FXII) is a multidomain serine protease that is the starter of intrinsic coagulation pathway. FXII deficiency and clinical hemostasis are not associated with bleeding, so investigators have not considered FXII important in physiology for a long time. In seeking explanation for...

SD Rat Liver Polydatin Concentration Control Based On Sliding Mode with High-gain Observer

Jian-Yang Lin, Yu-Shuai Bu, Jin-Rui Gao
In this paper, we use the sliding mode control based on high gain observer to control the concentration of Polydatin in the liver of SD rates. Through data processing and with the help of the nlinfit function in MATLAB, we take a fitting analysis by using the Polydatin distribution data in SD rats’ liver...

The Research on the Mechanism of Antioxidative and Growth-Promoting Effects of Polyphenols from the Involucres of Castanea Mollissima Blume on IEC-6 Cells

Y. Xiong, X. Zhao, S. Dong, K.J. Guo
The aim of the paper was to study the effects of Polyphenolic extract from involucres of Castanea mollissima Blume (PICB) on IEC-6 cell proliferation and growth and antioxidant status. The IEC-6 heat stress model was established and IEC-6 cells of control group and heat stress group were treated with...

Preparation and Characterization of Antimicrobial Activity Bacterial Cellulose Membrane

Wei-Hua Tang, Shi-Ru Jia, Yuan-Yuan Jia
Bacterial cellulose (BC) shows good potentiality as active packaging material. However, bacterial cellulose itself has no antimicrobial activity to prevent microbial contamination. To achieve antimicrobial activity, -poly lysine ( -PL) was incorporated into BC by immersing BC in -PL solution. Investigations...

Analysis on the Performance of the Balb/c 3T3 Two-stage Assay in a Miniature Culture Area and in a Perfusion Culture Model

Vania H. M. Teofilo, Jin-Tao Li, Xin-Hui Liu, Ru-Gang Zhong, Si Ye, Zheng-Bo Zhao, Yi Zeng
The Balb/c 3T3 cell transformation assay (CTA) is presently undergoing extensive pre-validation testing but its potential for routine chemical screening remains to be fully exploited due it being more costly and more time consuming than other CTAs. To downscale the assay 24-well plates were used to score...

Mitochondrial Impairment Mechanism in D-galactose-induced Senescence in Experimental Fibroblast Cell Model

Wei Ye, Jian-Ming Cao, Xiao-Jun Tang, Chu Liu, Dong-Jie Hao, Jing Jin, Jian-Xin Lu
To establish an effective model for cellular senescence, human embryo lung fibroblast cells (MRC-5) were cultured in D-galactose (D-Gal) medium respectively, as control, same concentration of D-glucose (D-Glu) was used. Decrease in cell proliferation, increase in senescence associated -galactosidase...

The Research of Small Molecule Soybean Polypeptide Precipitated by TCA

Jian Xiong, Yu-Ke Xiang, Jun Ye, Yu-Hao Zhang
Trichloroacetic acid (tca) makes macromolecular peptides expose more hydrophobic groups and have precipitation effect. It’s widely used in the desalination and concentration of protein and peptide. This experiment use the alkaline protease enzymolysis the soybean protein isolated and makes the products...

Parameters Identification of Short Hanger Based on Adaptive Genetic Algorithm

Pei Yuan
The hanger tension is crucial in arch bridges, and the fundamental frequency of short hangers is significantly affected by the flexural rigidity. In order to measure the hanger tension considering the flexural rigidity, the Additional Mass Method (AMM) is applied. The tension and flexural rigidity can...

A Rate-limited Step Guided by Different Regulation of Chromatin Modifiers Should be Undergone after Initiation Phase in Cellular Reprogramming

Rong Hu, Xian-Hua Dai, Zhi-Ming Dai, Qian Xiang
At early stage and late stage of cellular reprogramming the transgenes arouse two waves of transcription and epigenetic abruptly, while it is relatively quiet in intermediate stage. In this paper we analyzed the gene expression and chromatin modification data at different time point of mouse embryonic...

Novel Mercaptan Derivatives Nanogold Particles with Hyperchromic and Fluorescence Sensitization Effects

Dorothy Araba Yakoba Agyapong, Ran-Lin Zhao, Cai-Xia Li, Wen-Ju Du, Hong-Juan Zeng
Carcinoembryonic antigen (CEA), typically associated with certain tumors is widely used as clinical tumor markers for diagnosing certain cancers and hence its detection is of great essence in clinical diagnostics. A large number of surface atoms of nanoparticles have huge surplus bonding ability, because...

Clinical Analysis on the Use of the Photon Therapeutic Apparatus Combined with Peal Burn Ointment in Treatment for Breast Cancer Patients

Chun-Ni Fan, Ning Liu, Guang Sun
Objective: To investigate Carnation-87C photon therapeutic apparatus combined with peal burn ointment in the clinical effect of breast cancer patients after operation with unhealed incision of skin flap necrosis. Method: The treatment groups in the conventional treatment supplemented by photon therapeutic...

Clinical Application Research on the Use of Carnation Photon Therapeutic Apparatus in Treatment for Acute Postpartum Mastitis

Miao Miao, Chun-Ni Fan, Ning Liu, Guang Sun
Purpose: To investigate the clinical effect of photon therapeutic apparatus in the treatment of acute postpartum mastitis patients. Method: 48 cases of acute postpartum mastitis patients were randomly divided into two groups, treatment group and control group. Patients in the treatment group were treated...

The Expressions of HIF-1α, CA153, CEA and CA125 in Breast Cancer and Significance with Chemotherapy

Shou-Shui Yu, Feng-Liang Xu, Qiang Yin, Gang-Ping Wang, Shi-Cai Hou, Min Liu
Biomarker in tissue and serum plays significant roles in patient management and treatment and can be used as prognostic factors. For the diagnosis, treatment, prognosis study of breast cancer, we examined the expressions of Hypoxia inducible factor 1α (HIF-1α) in 128 cases mammary carcinoma tissues,...

Prognostic Significance of COX-2 and VEGF in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy

Gang-Ping Wang, Xue Wang, Zhao-Jun Ding, Yun-Ai Liang, Zuo-Feng Zhang
In recent years, the incidence of breast cancer is signifcantly on the rise all over the world, hence, how to treat breast cancer, correctively evaluate the prognosis and find the postoperative recurrence of patients with breast cancer have been paid attention by more and more scholars all over the world....

Analysis of Effect of the Position on Weighted Degree Kernel for Splice Site Prediction

Tian-Qi Wang, Yong Xu
Prediction of splice sites plays a key role in the annotation of genes. SVM with the weighted degree kernel has been proved to achieve a satisfactory performance. However, this kernel did not consider the effect of the position. In this article, we explored the relationship between the weighted degree...

Improvement of Image Characteristics in Digital Breast Tomosynthesis by Incorporating a Compressed-sensing (CS) Deblurring Framework: Simulation Study

Heemoon Cho, Kyuseok Kim, Hyosung Cho, Hyunwoo Lim, Yeonok Park
In this work, we considered a compressed-sensing (CS)-based framework with the total-variation regularization penalty for image deblurring of high accuracy in digital breast tomosynthesis (DBT). We implemented the proposed algorithm and performed a systematic simulation to demonstrate its viability for...

Theoretical Simulation and Experimental Study on the Crystal Morphology of L-arabinose

Biao Hu, Lei Liang, Ping-Jun Zhang, Shu-Juan Yu
The crystal morphology, growth faces as well as the structures of important facets of -L-arabinose were simulated by BFDH, growth morphology(GM) and equilibrium morphology(EM) methods in this paper. By measuring the attachment energy and lattice energy of the crystal, the relative growth rates and morphology...

The Clinical Medication Safety Based on Petri Net Monitoring

Zhi-Hui Huang
Pharmacovigilance and rational use of drugs, clinical application and evaluation, drug risk management is an important content of drug epidemiology. How to take advantage of Petri net monitoring Clinical Medication Safety in the hospital information system, obtain human reliability fault data, and using...

The Distilled Characteristics Analysis of Olive Brandy

Ji-Tian Song, Xi Chen, Hong Kan, Wen-Chao Wang, Yu-Chen Song, Wei Tian
There will be a lot of fragrant component loss and damage in the distillation process of olive brandy, which influences the quality of brandy seriously. In order to obtain good quality brandy, the effects of different distillation temperature are studied. It is found that the most suitable temperature...

Effects of Soil Amelioration on Photosynthetic Physiology and Soil Respiration of Blueberry

Xia Qiu, Yun-Peng Zhang, Kai-Li Chen, Zhi-Hui Wang, Xun Wang
The best soil for blueberries is a sandy loam that is well drained and contains lots of organic matter. And soil for blueberries should also be acidic. In order to modify the crop land to be fit for blueberry growth, we added several soil conditioners, peat, manure, commercial fertilizer slag, sawdust,...

Preparation and Properties of Crosslinked Silk Fibroin Film

Ya-Fei Wang, Chen Lu, Dan Long, Qiang Zhang, Shu-Qin Yan
In this study, regenerated silk fibroin (SF) film was prepared by using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as cross-linking agent and N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant agent. To evaluate the effects of EDC cross-linking agent...

The Status of Edible Mushrooms Resource Development and Nutrition Evaluation in Sichuan Province, China

Qian-Qian Tang, Yu-Xian You, Yi-Wen Li, Si-Qi Zeng, Di Chen, Zhao Su, Ai-Ping Liu, Hong Chen, Chao-Hui Feng, Yao-Wen Liu, Cheng Li, Yun-Tao Liu
With the development of our agriculture, the utilization of edible mushrooms is more and more widely recognized. The resources of them are abundant in our country, especially in Sichuan. They contain not only rich nutrients, but also active substances such as polysaccharides, which with the biological...

A Control Strategy of a Haptic Display Using Honeycomb

Ryota Ishibashi
This paper proposes a kind of tactile display using composite materials. Proposed display consists of a core honeycomb structure and a bi-elastic material. The system has both the endoskeltal and the exoskeltal properties. Then, it can realize an elastic display strategy for rendering softness based...

Significance of Combined Imaging BI-RADS Assessment in Breast Cancer and Pathology Comparison

Min Liu, Zhi-Hou Zhou, Ying Li, Jing-Zhong Xu, Gang-Ping Wang
Mammography (MG) is the only proven modality to reduce mortality of breast cancer and has been shown to reduce mortality from breast cancer, and ultrasonography (US) is a well-known adjunct to screening MG. BI-RADS classification is actually practical and correlation with histopathology on the palpable...

Clinical and Radiological Study of Adenoid Cystic Carcinoma in Salivary gland, Sinonasal Tract and Nasopharyngeal

Bo-Hong Shi, Yong-Mei Huang, Tao Qin
Adenoid cystic carcinoma (ACC) is one of the most common primary malignancies of the major and minor salivary glands. ACC is an uncommon neoplasm that most frequently arises in salivary glands and related tissue in the head and neck region. Unlike head and neck squamous cell carcinoma, ACC is characterized...

Analgesic Contrast of Diverse Administrations of Botulinum Neurotoxin A (BoTN/A) Using Rat Neuropathic Pain Model

Qiong-Fang Yang, Li-Zu Xiao, Wei-Jiao Xie, Yun-Hai Qiu
As a widely used clinical biomedicine for movement disorders and anti-wrinkle, in recent years, BoTN/A has emerged as a potential approach for treating refractory pain, especially migraine, diabetic pain, postherpetic neuralgia (PHN). In this study, we compared the analgesic effect of diverse administrations...

A Better Characterization of Brain Damage in Carbon Monoxide Intoxication Assessed in Vivo Using Diffusion Kurtosis Imaging

Wen-Yao Fan, Wen-Bin Zheng, Xi-Ran Chen
Diffusion kurtosis imaging findings in delayed encephalopathy after acute carbon monoxide poisoning have seldom reported. We present a study of a 25 -year-old female with acute carbon monoxide poisoning by using conventional magnetic resonance imaging (MRI) and diffusion kurtosis imaging (DKI). Conventional...

Diffusion Kurtosis Imaging Detects Acute Microstructural Changes in White and Gray Matter after Alcohol Intoxication in Rat

Xi-Ran Chen, Jie-Ying Zeng, Ling-Mei Kong, Wen-Bin Zheng
Diffusion kurtosis imaging (DKI) is an extension of Diffusion tensor imaging (DTI) but based on a non-gaussian diffusion model, allowing more comprehensive characterization of microstructural changes in the brain. The aim of this research was to test the technical feasibility to microstructural changes...

Application of Graph Theory Features for the Objective Diagnosis of Depressive Patients with or without Anxiety: an Rs-fMRI Study

Xiang-Yu Shen, Jing-Yu Zhu, Mao-Bin Wei, Jiao-Long Qin, Rui Yan, Qiu-Xiang Wei, Zhi-Jian Yao, Qing Lu
Purposes: To probe abnormality that may lead to anxiety in depressive patients. Procedures: This study investigated the graph theory features ahead of machine learning feature selection procedure. Classification methods were applied afterwards. Methods: Graph theory, statistical analysis and forward...

Altered Anatomical Modular Organization of Brain Networks in Patients with Major Depressive Disorder

Jing-Yu Zhu, Xiang-Yu Shen, Jiao-Long Qin, Mao-Bin Wei, Rui Yan, Jian-Huai Chen, Zhi-Jian Yao, Qing Lu
This study investigates the abnormal brain structural connectivity in patients with major depressive disorder (MDD) through a modularization approach. Diffusion tensor imaging (DTI) data were collected from 20 MDD patients and 20 age-matched controls. The structural modularity characteristics and their...

Synthesis of Novel pH-responsive PEGylated Zwitterionic Triblock Copolymer

Hai-Liang Dong, Zhong-Li Niu, Xiao-Ting Zhang, Shu-Yu Zhu, Ying Yao, Ya Gao, Bin Sun, Mei-Fang Zhu, Xiao-Ze Jiang
Well-defined PEGylated zwitterionic triblock copolymer, poly(ethylene glycol)-b-poly¬¬¬¬(methacrylic acid)-b-poly(2-aminoethyl methacrylate) (PEG-b-PMAA-b -PAEMA), was prepar¬e¬d from poly(ethylene glycol)-b-poly(tert-butyl meth acrylate) -b-poly(N-(tert-butoxycarbonyl)¬¬¬¬ amino ethyl methacrylate)(PEG-b-PtBocMA-b-PtBoc...

Quantification the Recombinant SUMO-psTnI in E.coli by SPE-HPLC

Tu Zhang, Qiu-Ling Xie, Yong Luo, Cai-Kun Wang
A simple and rapid reversed-phase liquid chromatography (RP-HPLC) method has been developed and validated for the quantification of recombinant SUMO-psTnI in E.coli BL21/PET-3C. This method could be used to monitor the target protein during fermentation and purification process. After Sonication in 20mMTris...

Surface Modification of Fe3O4 Nanoparticles for the Preparation of Magnetic Responsive Hydrogels

Peng-Hui Wang, Hai-Liang Dong, Shu-Yu Zhu, Zhong-Li Niu, Xiao-Ting Zhang, Bin Sun, Mei-Fang Zhu, Xiao-Ze Jiang
A novel approach was developed to synthesize polymerizable vinyl group functionalized Fe3O4 nanoparticles with high aqueous dispersion stability. Firstly, the Fe3O4 nanoparticles were synthesized via chemical co-precipitation method. Then, azide-functional Fe3O4 nanoparticles were obtained by a two-step...

Bio-Mechanically Active Ceramic-Polymeric Hybrid Scaffolds for Tissue Engineering

Aversa Raffaella, Sorrentino Roberto, Apicella Antonio
The research develops and tests new hybrid biomimetic materials that work as mechanically stimulating "scaffolds" to promote early regeneration in implanted bone healing phases. A biomimetic nanostructured osteo-conductive material coated apparatus is presented. A bio-inspired approach to materials and...

Digestion Mechanism Analysis of Total Arsenic Determination in Animal Origin Seafood by Atomic Fluorescence Spectrometry

Yong-Fu Shi, Qian-Yun Zhan, Hui-Juan Yu, Run-Run Gu, Dong-Mei Huang, Yuan Wang, You-Qiong Cai
In the present study, canned crab and canned tuna were taken as the quality control samples, and microwave digestion combined with wet-digestion was estimated as the optimum digestion method for animal origin seafood after comparing with the microwave digestion. The quality control samples were digested...

Wave Analysis of a Diffusive Modified Leslie-Gower Predator-prey System with Holling Type IV Schemes

Jiang-Lin Zhao, Ding-Sheng Deng
A diffusive predator-prey model with modified Leslie-Gower and Holling type IV schemes is investigated analytically and numerically. Mathematical theoretical works mainly focus on the existence of traveling wave solutions. Numerical simulations are performed to confirm the feasibility of traveling wave...

Effects of Oxalic Acid on Physiological-biochemical Traits of Cold Temperate Larix gmelinii Seedlings under Soil Nutrient Deficiency

Fu-Xing Cui, Jin-Feng Song, Dao-Guang Zhu, Jin-Bo Li, Zhen-Yang Qi, Lin Shan, Hong-Wei Ni
Organic acids obviously affect plant survival and growth under soil nutrient deficiency condition. Variable proportion A1 and C horizon mixed cold temperate brown coniferous forest soils was applied to make soil nutrient deficiency condition, and then Larix gmelinii seedlings were planted in these soils....

PCV Measurement for Biomass Assessment in Suspension Culture of Insect Cells

Dong-Chen Liu, Qiu-Ling Xie
In this study, we present a new approach for biomass assessment in insect cell culture using a disposable microcentrifuge tube, which is composed with an upper chamber for sample loading and a lower 5 L capillary for cell collection during centrifugation. The height of the cell pellet within the capillary...