Proceedings of the 2016 International Conference on Biological Sciences and Technology

Mitochondrial Impairment Mechanism in D-galactose-induced Senescence in Experimental Fibroblast Cell Model

Authors
Wei Ye, Jian-Ming Cao, Xiao-Jun Tang, Chu Liu, Dong-Jie Hao, Jing Jin, Jian-Xin Lu
Corresponding Author
Wei Ye
Available Online January 2016.
DOI
https://doi.org/10.2991/bst-16.2016.18How to use a DOI?
Keywords
D-galactose, Mitochondria, Oxidative Stress, Oxidative Phosphorylation.
Abstract
To establish an effective model for cellular senescence, human embryo lung fibroblast cells (MRC-5) were cultured in D-galactose (D-Gal) medium respectively, as control, same concentration of D-glucose (D-Glu) was used. Decrease in cell proliferation, increase in senescence associated -galactosidase activity, up-expression of p21 protein, and cell cycle arrest at S-phage were observed in D-Gal-treated cells. Meanwhile, D-Gal-treated cells showed the significant increased ROS and MDA level and decreased SOD activity. Furthermore, mitochondrial impairment and decrease in efficiency of oxidative phosphorylation (OXPHOS) was induced by D-Gal as evidenced by the decreased transmembrane potential, reduction of ATP production and changes of respiration function. Additionally, the significant decrease of mitochondrial quantity, mitochondrial DNA (mtDNA) copy number and increased mtDNA damage were detected in D-Gal-treated cells. Our data demonstrate that D-Gal induces mitochondrial oxidative impairment associated with increased generation of ROS, ultimately inhibiting the ATP synthesis which contributes to premature cellular senescence.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
The International Conference on Biological Sciences and Technology
Part of series
Advances in Biological Sciences Research
Publication Date
January 2016
ISBN
978-94-6252-161-2
ISSN
2468-5747
DOI
https://doi.org/10.2991/bst-16.2016.18How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Wei Ye
AU  - Jian-Ming Cao
AU  - Xiao-Jun Tang
AU  - Chu Liu
AU  - Dong-Jie Hao
AU  - Jing Jin
AU  - Jian-Xin Lu
PY  - 2016/01
DA  - 2016/01
TI  - Mitochondrial Impairment Mechanism in D-galactose-induced Senescence in Experimental Fibroblast Cell Model
BT  - The International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.18
DO  - https://doi.org/10.2991/bst-16.2016.18
ID  - Ye2016/01
ER  -