To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus
- Ying-Ying Liu, Jing Wang
- Corresponding Author
- Ying-Ying Liu
Available Online January 2016.
- https://doi.org/10.2991/bst-16.2016.4How to use a DOI?
- Hemocytometer, Measurement uncertainty, White blood cell.
- Acridine orange (AO) and ethidium bromide (EB) are two fluorescent dyes for cell nuclei staining. The nucleus images by these two staining can make it easy to differentiate white blood cell (WBC) from red blood cell (RBC). We applied this principle to the white blood cell count by hemocytometer and this can eliminate the errors caused by misjudging of WBC. At the same time, microscopic imaging can improve the accuracy of the volume measurement. The extended relative uncertainty of two measurements have been analyzed and compared.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ying-Ying Liu AU - Jing Wang PY - 2016/01 DA - 2016/01 TI - To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus BT - The International Conference on Biological Sciences and Technology PB - Atlantis Press SN - 2468-5747 UR - https://doi.org/10.2991/bst-16.2016.4 DO - https://doi.org/10.2991/bst-16.2016.4 ID - Liu2016/01 ER -