Proceedings of the 2016 International Conference on Biological Sciences and Technology

To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus

Authors
Ying-Ying Liu, Jing Wang
Corresponding Author
Ying-Ying Liu
Available Online January 2016.
DOI
https://doi.org/10.2991/bst-16.2016.4How to use a DOI?
Keywords
Hemocytometer, Measurement uncertainty, White blood cell.
Abstract
Acridine orange (AO) and ethidium bromide (EB) are two fluorescent dyes for cell nuclei staining. The nucleus images by these two staining can make it easy to differentiate white blood cell (WBC) from red blood cell (RBC). We applied this principle to the white blood cell count by hemocytometer and this can eliminate the errors caused by misjudging of WBC. At the same time, microscopic imaging can improve the accuracy of the volume measurement. The extended relative uncertainty of two measurements have been analyzed and compared.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
The International Conference on Biological Sciences and Technology
Part of series
Advances in Biological Sciences Research
Publication Date
January 2016
ISBN
978-94-6252-161-2
ISSN
2468-5747
DOI
https://doi.org/10.2991/bst-16.2016.4How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Ying-Ying Liu
AU  - Jing Wang
PY  - 2016/01
DA  - 2016/01
TI  - To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus
BT  - The International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.4
DO  - https://doi.org/10.2991/bst-16.2016.4
ID  - Liu2016/01
ER  -