Proceedings of the 2016 International Conference on Biological Sciences and Technology

Preparation and Properties of Crosslinked Silk Fibroin Film

Authors
Ya-Fei Wang, Chen Lu, Dan Long, Qiang Zhang, Shu-Qin Yan
Corresponding Author
Ya-Fei Wang
Available Online January 2016.
DOI
https://doi.org/10.2991/bst-16.2016.33How to use a DOI?
Keywords
Silk fibroin, Film, Crosslinking, Structure, Properties.
Abstract
In this study, regenerated silk fibroin (SF) film was prepared by using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as cross-linking agent and N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant agent. To evaluate the effects of EDC cross-linking agent on SF film performence, the water resistance, condensed structure, tensile strength, elongation, Young’s modulus of crosslinked film were examined and analyzed. The water solubility and Young’s modulus of the film with 20 wt% EDC decreased remarkably, while film elongation increased. Further, the FTIR, Raman spectra and X-Ray Diffraction (XRD) were employed to investigate the molecule conformation transition and insight the mechanism of the crosslinking. The results demonstrated that crosslinked film contained more silk I when ~20 wt% EDC (weight ratio to SF) was added. When more than 20 wt%, the content of silk I decreased remarkably while that of silk II increased in the SF film. Due to the reaction of EDC, carboxylic acid groups and amino groups were bound by amide bond formation between the SF molecules. The NHS plays an activation of -OH group; the MES creates a faintly acid condition to be beneficial for the cross-link reaction. This study provided an important method to prepare insoluble SF materials and promoted application of the SF materials.
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Proceedings
The International Conference on Biological Sciences and Technology
Part of series
Advances in Biological Sciences Research
Publication Date
January 2016
ISBN
978-94-6252-161-2
ISSN
2468-5747
DOI
https://doi.org/10.2991/bst-16.2016.33How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Ya-Fei Wang
AU  - Chen Lu
AU  - Dan Long
AU  - Qiang Zhang
AU  - Shu-Qin Yan
PY  - 2016/01
DA  - 2016/01
TI  - Preparation and Properties of Crosslinked Silk Fibroin Film
BT  - The International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.33
DO  - https://doi.org/10.2991/bst-16.2016.33
ID  - Wang2016/01
ER  -