Preparation and Properties of Crosslinked Silk Fibroin Film

DOI

10.2991/bst-16.2016.33How to use a DOI?

Keywords

Silk fibroin, Film, Crosslinking, Structure, Properties.

Abstract

In this study, regenerated silk fibroin (SF) film was prepared by using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as cross-linking agent and N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant agent. To evaluate the effects of EDC cross-linking agent on SF film performence, the water resistance, condensed structure, tensile strength, elongation, Young’s modulus of crosslinked film were examined and analyzed. The water solubility and Young’s modulus of the film with 20 wt% EDC decreased remarkably, while film elongation increased. Further, the FTIR, Raman spectra and X-Ray Diffraction (XRD) were employed to investigate the molecule conformation transition and insight the mechanism of the crosslinking. The results demonstrated that crosslinked film contained more silk I when ~20 wt% EDC (weight ratio to SF) was added. When more than 20 wt%, the content of silk I decreased remarkably while that of silk II increased in the SF film. Due to the reaction of EDC, carboxylic acid groups and amino groups were bound by amide bond formation between the SF molecules. The NHS plays an activation of -OH group; the MES creates a faintly acid condition to be beneficial for the cross-link reaction. This study provided an important method to prepare insoluble SF materials and promoted application of the SF materials.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ya-Fei Wang
AU  - Chen Lu
AU  - Dan Long
AU  - Qiang Zhang
AU  - Shu-Qin Yan
PY  - 2016/01
DA  - 2016/01
TI  - Preparation and Properties of Crosslinked Silk Fibroin Film
BT  - Proceedings of the 2016 International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SP  - 215
EP  - 222
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.33
DO  - 10.2991/bst-16.2016.33
ID  - Wang2016/01
ER  -