Preparation and Properties of Crosslinked Silk Fibroin Film
- Ya-Fei Wang, Chen Lu, Dan Long, Qiang Zhang, Shu-Qin Yan
- Corresponding Author
- Ya-Fei Wang
Available Online January 2016.
- https://doi.org/10.2991/bst-16.2016.33How to use a DOI?
- Silk fibroin, Film, Crosslinking, Structure, Properties.
- In this study, regenerated silk fibroin (SF) film was prepared by using 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as cross-linking agent and N-hydroxysuccinimide (NHS), 2-morpholinoethanesulfonic acid (MES) as assistant agent. To evaluate the effects of EDC cross-linking agent on SF film performence, the water resistance, condensed structure, tensile strength, elongation, Young’s modulus of crosslinked film were examined and analyzed. The water solubility and Young’s modulus of the film with 20 wt% EDC decreased remarkably, while film elongation increased. Further, the FTIR, Raman spectra and X-Ray Diffraction (XRD) were employed to investigate the molecule conformation transition and insight the mechanism of the crosslinking. The results demonstrated that crosslinked film contained more silk I when ~20 wt% EDC (weight ratio to SF) was added. When more than 20 wt%, the content of silk I decreased remarkably while that of silk II increased in the SF film. Due to the reaction of EDC, carboxylic acid groups and amino groups were bound by amide bond formation between the SF molecules. The NHS plays an activation of -OH group; the MES creates a faintly acid condition to be beneficial for the cross-link reaction. This study provided an important method to prepare insoluble SF materials and promoted application of the SF materials.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ya-Fei Wang AU - Chen Lu AU - Dan Long AU - Qiang Zhang AU - Shu-Qin Yan PY - 2016/01 DA - 2016/01 TI - Preparation and Properties of Crosslinked Silk Fibroin Film BT - The International Conference on Biological Sciences and Technology PB - Atlantis Press SN - 2468-5747 UR - https://doi.org/10.2991/bst-16.2016.33 DO - https://doi.org/10.2991/bst-16.2016.33 ID - Wang2016/01 ER -