Proceedings of the 2016 International Conference on Biological Sciences and Technology

PCV Measurement for Biomass Assessment in Suspension Culture of Insect Cells

Authors
Dong-Chen Liu, Qiu-Ling Xie
Corresponding Author
Dong-Chen Liu
Available Online January 2016.
DOI
https://doi.org/10.2991/bst-16.2016.50How to use a DOI?
Keywords
PCV measurement, Biomass assessment, Insect cell culture.
Abstract
In this study, we present a new approach for biomass assessment in insect cell culture using a disposable microcentrifuge tube, which is composed with an upper chamber for sample loading and a lower 5 L capillary for cell collection during centrifugation. The height of the cell pellet within the capillary is measured and expressed as the packed cell volume (PCV). PCV method showed an excellent correlation with cell counting measurement, but with lower average of the errors (3.3%) compared to manual cell counting (12.4%). PCV method was shown to be statistically reliable and more precise, rapid. And it also demonstrates the potential use of this method to monitor the changes in insect cell culture that can affect cell size, for example as seen under baculovirus infection.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
The International Conference on Biological Sciences and Technology
Part of series
Advances in Biological Sciences Research
Publication Date
January 2016
ISBN
978-94-6252-161-2
ISSN
2468-5747
DOI
https://doi.org/10.2991/bst-16.2016.50How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Dong-Chen Liu
AU  - Qiu-Ling Xie
PY  - 2016/01
DA  - 2016/01
TI  - PCV Measurement for Biomass Assessment in Suspension Culture of Insect Cells
BT  - The International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.50
DO  - https://doi.org/10.2991/bst-16.2016.50
ID  - Liu2016/01
ER  -