The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)

Molecular Damage Evaluation and Chromosome Aberration in Blood Lymphocyte of Medical Radiation Workers

Authors
Yanti Lusiyanti, Masnelly Lubis, Suryadi, Sardini, Sofiati Purnami, Nastiti Rahajeng, Darlina, Siti Nurhayati
Corresponding Author
Yanti Lusiyanti
Available Online 8 June 2020.
DOI
https://doi.org/10.2991/ahsr.k.200523.082How to use a DOI?
Keywords
medical workers, dicentrics, MN, DNA damages, Comet assay
Abstract

A preliminary evaluation on the cytogenetic effects and DNA damages was done in blood of twenty two person at the age between 30-56 years old from medical workers as expose group and their matched twenty from administration workers (control). Their bloods were collected with their full informed consent and culture set up for cytogenetic evaluation as well as nucleic acid damage observation with comet assay that were done according to the standard procedures. Lymphocytes were scored, either by using analysis of manual observation and automatically with metaphase finder, for the presence of chromosomal aberration and Mitotik Index. The result show that the Mitotik Index on expose group were no significant differences between exposure group and control. The frequencies of disentric chromosomes both in expose group and control were not found. Evaluation of DNA damage observation using Comet assay showed no any effects observed in lymphocytes for both study and control groups. Our data did not reveal any significant difference in expose group compared to control level in parameters by mean cytogenetics biomarkers studied which is supported by the Comet evaluation.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
Series
Advances in Health Sciences Research
Publication Date
8 June 2020
ISBN
978-94-6252-976-2
ISSN
2468-5739
DOI
https://doi.org/10.2991/ahsr.k.200523.082How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Yanti Lusiyanti
AU  - Masnelly Lubis
AU  - Suryadi
AU  - Sardini
AU  - Sofiati Purnami
AU  - Nastiti Rahajeng
AU  - Darlina
AU  - Siti Nurhayati
PY  - 2020
DA  - 2020/06/08
TI  - Molecular Damage Evaluation and Chromosome Aberration in Blood Lymphocyte of Medical Radiation Workers
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 342
EP  - 347
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.082
DO  - https://doi.org/10.2991/ahsr.k.200523.082
ID  - Lusiyanti2020
ER  -