The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)

In Silico Study of Single Chain Fragment Variable (ScFv) on Chikungunya Virus Using Indonesian Strain

Authors
Korry Novitriani, Shabarni Gaffar, Bacthi Alisjahbana, Umi Baroroh, Ade Rizki, Muhammad Yusuf, Toto Subroto
Corresponding Author
Toto Subroto
Available Online 8 June 2020.
DOI
https://doi.org/10.2991/ahsr.k.200523.008How to use a DOI?
Keywords
silico, CHIKV, protein envelope E1E2, anti E1E2 scFv mutation
Abstract

Objective: Because so far the paper-based commercial chikungunya tool in Indonesia has a very low sensitivity of only 20.5-50.8%, and this is supportive because of the different use of strain in the diagnostic tool so the purpose of this research is to study the selectivity of sampling from Indonesian strains. The CHIKVE1E2 model was obtained from NCBI with anti E1E2scFv based on global energy parameters in silico. Method: The selected ScFv was modified through the replacement of amino acids that play a role in interaction with scFv which is more selective towards E1E2 with lower molecular interaction energy. Docking is done using the PatchDock and FireDock programs. Visualization is done using Biovia Discovery Studio (BDS). Results and discussion: Research shows that modified scfv of y232s and y232t respectfully provides global energy values of -58.59 and -45.42 kcal / mol; VDW draws -32.79 and -29.70 kcal / mol; VDW repulsive 19.75 and 15.50 kcal / mol; ACE 1.69 and 0.65 kcal / mol and HB -5.67 and-5.79 kcal / mol. Conclusion: a method for predicting interactions between CHIKV E1E2 envelope protein and scFv needs to be further developed to get better results.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
Series
Advances in Health Sciences Research
Publication Date
8 June 2020
ISBN
978-94-6252-976-2
ISSN
2468-5739
DOI
https://doi.org/10.2991/ahsr.k.200523.008How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Korry Novitriani
AU  - Shabarni Gaffar
AU  - Bacthi Alisjahbana
AU  - Umi Baroroh
AU  - Ade Rizki
AU  - Muhammad Yusuf
AU  - Toto Subroto
PY  - 2020
DA  - 2020/06/08
TI  - In Silico Study of Single Chain Fragment Variable (ScFv) on Chikungunya Virus Using Indonesian Strain
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 25
EP  - 29
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.008
DO  - https://doi.org/10.2991/ahsr.k.200523.008
ID  - Novitriani2020
ER  -