Identification of a Light-Harvesting Chlorophyll a/b-Binding Protein Gene in Gardenia jasminoides
- DOI
- 10.2991/cmfe-15.2015.34How to use a DOI?
- Keywords
- Light-harvesting chlorophyll a/b-binding protein (LHCB); Gardenia jasminoides; photosynthesis component; cDNA library; gene cloning.
- Abstract
Objective: The major light-harvesting complex of photosystem II , Lhcbs, are assembled in its trimeric form, equipped with chlorophyll and xanthophylls, embedded in the thylakoid membrane of green plants, and play critical roles in harvesting light energy for photosynthesis. The objective of this work is to isolate a gene of Lhcb in Gardenia jasminoides. Methods: A Gardenia jasminoides fruit cDNA library was constructed, and the GjLhcb cDNA was isolated from the cDNA library by sequencing method. Results: The GjLhcb cDNA is 946bp, contains a predicted 795bp open reading frame that encodes 265 amino acids. A phylogenetic analysis was conducted with previously characterized Lhcbs from other plant species. A three-dimensional trimers model of GjLhcb was built. Conclusion: The phylogenetic analysis suggested that the GjLhcb has a chloroplast transit peptide, the mature form has 230 amino acids; and contains an N-terminal Thr as a potential phosphorylation site, which is important in regulation of state transitions. The three-dimensional structure of GjLhcb is similar with the structure of Lhcb1 in Spinacia oleracea. This is the first Lhcb identified in Gardenia jasminoides.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Lan Gao AU - Hao-Ming Li PY - 2015/07 DA - 2015/07 TI - Identification of a Light-Harvesting Chlorophyll a/b-Binding Protein Gene in Gardenia jasminoides BT - Proceedings of the International Conference on Chemical, Material and Food Engineering PB - Atlantis Press SP - 142 EP - 145 SN - 2352-5401 UR - https://doi.org/10.2991/cmfe-15.2015.34 DO - 10.2991/cmfe-15.2015.34 ID - Gao2015/07 ER -