Thermal Simulation for Continuous-flow PCR System
Shenglan Zeng, Bingyu Chen, Lingfeng Cai, Quanjun Liu
Available Online August 2016.
- https://doi.org/10.2991/cset-16.2016.64How to use a DOI?
- PCR, microfluidic, COMSOL, Simulation
- A continuous-flow PCR system is presented, which based on the microfluidic platform briefly consists of a PMMA chip with a PCR liquid channel, three heating units. Each of them contained a heat conducting block on a commercially available thin PI substrate. In order to ensure temperature uniformity across the PCR temperature zones and minimal cross-talk between the zones, the design of chip and heating units was assisted by COMSOL. The simulation results presented the most suitable thickness for the chip and heat conducting block as well as the height limit for the liquid channel. Within the optimized parameters, our system not only has the advantages of fast amplification rates, but also has the benefits of small thermal mass and low power consumption.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Shenglan Zeng AU - Bingyu Chen AU - Lingfeng Cai AU - Quanjun Liu PY - 2016/08 DA - 2016/08 TI - Thermal Simulation for Continuous-flow PCR System BT - 2016 International Conference on Computer Science and Electronic Technology PB - Atlantis Press SP - 273 EP - 276 SN - 2352-538X UR - https://doi.org/10.2991/cset-16.2016.64 DO - https://doi.org/10.2991/cset-16.2016.64 ID - Zeng2016/08 ER -