Quantum-dot-based immunofluorescent imaging of Cancer Marker P53 Detection in Breast Cancer

DOI

10.2991/emcpe-16.2016.122How to use a DOI?

Keywords

Quantum dots; AgInS2/ZnS;Breast cancer; P53; Probes

Abstract

The study is to design a biomarker based on hydrophilic quantum dots(QDs) can be bound to the P53 protein for diagnoses of breast cancer (BC).To achieve the purpose, we uses the water-soluble quantum (QDs) AgInS2/ZnS connected with P53 monoclonal antibody to design a novel kind of biomarker as a new detection of breast cancer. Significantly, this targeted quantum dots not only have good target recognition ability, but also have low toxicity and strong light stability. Moreover, confocal laser scanning microscopy (CLSM) showed that the localized fluorescence would be detected around the nucleus of human breast cancer cell MCF7 (high expression of P53) compared with that of T47D (low expression of P53). In summary, we believe that this QDs probe will provide a broad platform for clinical diagnosis and treatment of breast cancer and in vitro studies in future.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Wanying Xing
AU  - Qiang Li
AU  - Xing Ren
AU  - Guang Sun
PY  - 2016/08
DA  - 2016/08
TI  - Quantum-dot-based immunofluorescent imaging of Cancer Marker P53 Detection in Breast Cancer
BT  - Proceedings of the 2016 5th International Conference on Environment, Materials, Chemistry and Power Electronics
PB  - Atlantis Press
SP  - 416
EP  - 421
SN  - 2352-5401
UR  - https://doi.org/10.2991/emcpe-16.2016.122
DO  - 10.2991/emcpe-16.2016.122
ID  - Xing2016/08
ER  -