HMGA2 Gene Silencing Suppression SW480 Colon Cancer Cell Invasion and Expression of Survivin
- 10.2991/emim-15.2015.24How to use a DOI?
- RNA interference; Survivin; HMGA2; p53
Objective To investigate the effects of high mobility group protein A2 (HMGA2) gene interference SW480 cell invasion, and to explore its interference with the expression of Survivin gene and its mechanism.Method: To construct the recombinant HMGA2 small hairpin interfering RNA expression vector pGenesil-1.1- HMGA2, SW480 colon cancer cells were transfected, cell growth were detected by MTT, the changing of cell invasion ability were detected by Transwell chamber invasion assay, The mRNA and protein expression of Survivin, hTERT and P53 were detected by PCR and Western-blot. Construction of RNA interference vectors which target P53 gene ,transfection SW480 cells ,detect the mRNA and protein expression of Survivin Result: After the HMGA2 gene interference, SW480 cell proliferation was significantly inhibited, inhibition rate was 34.44%, cell invasion was significantly inhibited, The mRNA and protein expression levels of Survivin and P53 genes significantly reduced. After P53 gene is disturbed, mRNA and protein expression levels of Survivin gene decline. Conclusion: Targeting and silencing HMGA2 gene have significantly inhibition for SW480 cells, while down Survivin gene expression, which may be related to down-regulation of gene expression in P53.
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Ping Wang AU - Shou xun Wang AU - Jun hong Dong PY - 2015/04 DA - 2015/04 TI - HMGA2 Gene Silencing Suppression SW480 Colon Cancer Cell Invasion and Expression of Survivin BT - Proceedings of the 2015 International Conference on Education, Management, Information and Medicine PB - Atlantis Press SP - 121 EP - 125 SN - 2352-5428 UR - https://doi.org/10.2991/emim-15.2015.24 DO - 10.2991/emim-15.2015.24 ID - Wang2015/04 ER -