Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018)

Substitution VW Medium Using Palm Date Puree In The Tissue Culture Of Grammatophyllum Scriptum

Authors
Etty Handayani, Gatot Supangkat, Anna Pangestuti
Corresponding Author
Etty Handayani
Available Online November 2018.
DOI
https://doi.org/10.2991/fanres-18.2018.38How to use a DOI?
Keywords
in vitro culture, orchid, plant puree, subculture
Abstract
The research aimed to determine date palm pure concentration and its effect on Tissue Culture of Grammatophyllum scriptum. The research used an experimental method with single factor and seven treatments arranged in a Completely Randomized Design. The treatments were: (1) Vacin & Went (VW) medium; (2) 50g/L date palm puree; (3) 100g/L date palm puree ; (4) 150g/L date palm puree; (5) 50g/L date palm puree + 30g/L sugar cane; (6) 100g/L date palm puree + 30g/L sugar cane; (7) 150g/date palm puree L + 30g/L sugar cane. All date palm puree treatments were combined with 3g/L foliar fertilizer. The observed variables were plant heights, number of leaves, number of shoots, the percentage of contamination, browning, life, and rooted explants. The result showed the growth and living performance of G. scriptum in the medium of 50g/L dates palm puree with and without sugar was not significantly different on the VW medium. It indicated that the growth of G. scriptum in this combination of such media was relatively similar with those grown on VW medium. The medium of 50g/L dates palm puree combined with 3g/L foliar fertilizer could substitute the VW medium in the subculture of G. scriptum
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Proceedings
Part of series
Advances in Engineering Research
Publication Date
November 2018
ISBN
978-94-6252-619-8
ISSN
2352-5401
DOI
https://doi.org/10.2991/fanres-18.2018.38How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Etty Handayani
AU  - Gatot Supangkat
AU  - Anna Pangestuti
PY  - 2018/11
DA  - 2018/11
TI  - Substitution VW Medium Using Palm Date Puree In The Tissue Culture Of Grammatophyllum Scriptum
PB  - Atlantis Press
SP  - 184
EP  - 186
SN  - 2352-5401
UR  - https://doi.org/10.2991/fanres-18.2018.38
DO  - https://doi.org/10.2991/fanres-18.2018.38
ID  - Handayani2018/11
ER  -