Substitution VW Medium Using Palm Date Puree In The Tissue Culture Of Grammatophyllum Scriptum
Etty Handayani, Gatot Supangkat, Anna Pangestuti
Available Online November 2018.
- https://doi.org/10.2991/fanres-18.2018.38How to use a DOI?
- in vitro culture, orchid, plant puree, subculture
- The research aimed to determine date palm pure concentration and its effect on Tissue Culture of Grammatophyllum scriptum. The research used an experimental method with single factor and seven treatments arranged in a Completely Randomized Design. The treatments were: (1) Vacin & Went (VW) medium; (2) 50g/L date palm puree; (3) 100g/L date palm puree ; (4) 150g/L date palm puree; (5) 50g/L date palm puree + 30g/L sugar cane; (6) 100g/L date palm puree + 30g/L sugar cane; (7) 150g/date palm puree L + 30g/L sugar cane. All date palm puree treatments were combined with 3g/L foliar fertilizer. The observed variables were plant heights, number of leaves, number of shoots, the percentage of contamination, browning, life, and rooted explants. The result showed the growth and living performance of G. scriptum in the medium of 50g/L dates palm puree with and without sugar was not significantly different on the VW medium. It indicated that the growth of G. scriptum in this combination of such media was relatively similar with those grown on VW medium. The medium of 50g/L dates palm puree combined with 3g/L foliar fertilizer could substitute the VW medium in the subculture of G. scriptum
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Etty Handayani AU - Gatot Supangkat AU - Anna Pangestuti PY - 2018/11 DA - 2018/11 TI - Substitution VW Medium Using Palm Date Puree In The Tissue Culture Of Grammatophyllum Scriptum PB - Atlantis Press SP - 184 EP - 186 SN - 2352-5401 UR - https://doi.org/10.2991/fanres-18.2018.38 DO - https://doi.org/10.2991/fanres-18.2018.38 ID - Handayani2018/11 ER -