Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018)

Antioxidant Activity of Protein Hydrolysates Derived from Javanese Freshwater Fishes

Authors
Yuli Witono, Iwan Taruna, Maryanto Maryanto, Riska Rian Fauziah, Wulan Suci Wahyuningtyas
Corresponding Author
Yuli Witono
Available Online November 2018.
DOI
https://doi.org/10.2991/fanres-18.2018.44How to use a DOI?
Keywords
Amino Acid, Antioxidant Activity, Javanese Freshwater Fishes, Protein Hydrolysates.
Abstract
Fish protein hydrolysates have been identified as the rich source of bioactive peptides. Thus, this study aims to determine the antioxidant activity of protein hydrolysates derived from common barb (Rasbora jacobsoni) and java carp (Barbonymus gonionotus). Therefore, in this study, two types of freshwater fishes were evaluated as a raw material to obtain protein hydrolysates exhibiting antioxidant activity. The fish’s protein was hydrolyzed by biduri protease with a concentration of 1, 2 and 3% (v/w) for 3 hours. Afterwards, the resulted dry hydrolysates were observed to determine the degree of hydrolysis, antioxidant activity (DPPH and reducing power), amino acid composition, and molecular weight. The result shows that the protein hydrolysates derived from common barb and java carp fish had percent value of inhibition (%RSA) respectively of 42.70% and 53.21%, an IC50 value of 1159.76 ppm and 935.84 ppm, reducing power value of 0.508 and 0.524. The protein hydrolysates derived from java carp fish had 15 kinds of an amino acid such as glutamic acid, aspartic acid and lysine, respectively of 13.34%; 8.36%; and 8.20%. The molecular weight distribution of protein hydrolysates derived from java carp fish had a value of about 23.09 kDa; 59.08 kDa
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
Part of series
Advances in Engineering Research
Publication Date
November 2018
ISBN
978-94-6252-619-8
ISSN
2352-5401
DOI
https://doi.org/10.2991/fanres-18.2018.44How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yuli Witono
AU  - Iwan Taruna
AU  - Maryanto Maryanto
AU  - Riska Rian Fauziah
AU  - Wulan Suci Wahyuningtyas
PY  - 2018/11
DA  - 2018/11
TI  - Antioxidant Activity of Protein Hydrolysates Derived from Javanese Freshwater Fishes
PB  - Atlantis Press
SP  - 213
EP  - 217
SN  - 2352-5401
UR  - https://doi.org/10.2991/fanres-18.2018.44
DO  - https://doi.org/10.2991/fanres-18.2018.44
ID  - Witono2018/11
ER  -