Antioxidant Activity of Protein Hydrolysates Derived from Javanese Freshwater Fishes
Yuli Witono, Iwan Taruna, Maryanto Maryanto, Riska Rian Fauziah, Wulan Suci Wahyuningtyas
Available Online November 2018.
- https://doi.org/10.2991/fanres-18.2018.44How to use a DOI?
- Amino Acid, Antioxidant Activity, Javanese Freshwater Fishes, Protein Hydrolysates.
- Fish protein hydrolysates have been identified as the rich source of bioactive peptides. Thus, this study aims to determine the antioxidant activity of protein hydrolysates derived from common barb (Rasbora jacobsoni) and java carp (Barbonymus gonionotus). Therefore, in this study, two types of freshwater fishes were evaluated as a raw material to obtain protein hydrolysates exhibiting antioxidant activity. The fish’s protein was hydrolyzed by biduri protease with a concentration of 1, 2 and 3% (v/w) for 3 hours. Afterwards, the resulted dry hydrolysates were observed to determine the degree of hydrolysis, antioxidant activity (DPPH and reducing power), amino acid composition, and molecular weight. The result shows that the protein hydrolysates derived from common barb and java carp fish had percent value of inhibition (%RSA) respectively of 42.70% and 53.21%, an IC50 value of 1159.76 ppm and 935.84 ppm, reducing power value of 0.508 and 0.524. The protein hydrolysates derived from java carp fish had 15 kinds of an amino acid such as glutamic acid, aspartic acid and lysine, respectively of 13.34%; 8.36%; and 8.20%. The molecular weight distribution of protein hydrolysates derived from java carp fish had a value of about 23.09 kDa; 59.08 kDa
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Yuli Witono AU - Iwan Taruna AU - Maryanto Maryanto AU - Riska Rian Fauziah AU - Wulan Suci Wahyuningtyas PY - 2018/11 DA - 2018/11 TI - Antioxidant Activity of Protein Hydrolysates Derived from Javanese Freshwater Fishes PB - Atlantis Press SP - 213 EP - 217 SN - 2352-5401 UR - https://doi.org/10.2991/fanres-18.2018.44 DO - https://doi.org/10.2991/fanres-18.2018.44 ID - Witono2018/11 ER -