Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein
- https://doi.org/10.2991/icadme-15.2015.24How to use a DOI?
- Aptasensor, structure-switching, hairpin probe, electrochemical, PDGF-BB
Using platelet derived growth factor B chain (PDGF-BB) as the model analyte, a structure-switching hairpin probe based electrochemical biosensing strategy for highly sensitive detection of protein is proposed in this work. Aptamer probe hybridized with the immobilized hairpin structured signal probe to form DNA duplex. Upon binding with target protein, competition between target molecule and the signal probe with the aptamer probe happened, inducing the signal probe from stretched duplex to hairpin structure. Under optimum experimental conditions, the target protein can be sensitively detected in a linear dynamic range from 0.1 ng/mL to 500 ng/mL with a low detection limit of 0.08 ng/mL.
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xia Hu AU - Song-Bai Zhang AU - Ji-Lin Lu AU - Li-Ying Zheng AU - Hong-En Geng AU - Yan-Qing Qiu AU - Yan-Yan Huang AU - Li-Ping Qiu AU - Yuan-Dao Chen PY - 2015/10 DA - 2015/10 TI - Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein BT - Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering PB - Atlantis Press SP - 114 EP - 117 SN - 2352-5401 UR - https://doi.org/10.2991/icadme-15.2015.24 DO - https://doi.org/10.2991/icadme-15.2015.24 ID - Hu2015/10 ER -