Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein

Authors
Xia Hu, Song-Bai Zhang, Ji-Lin Lu, Li-Ying Zheng, Hong-En Geng, Yan-Qing Qiu, Yan-Yan Huang, Li-Ping Qiu, Yuan-Dao Chen
Corresponding Author
Xia Hu
Available Online October 2015.
DOI
https://doi.org/10.2991/icadme-15.2015.24How to use a DOI?
Keywords
Aptasensor, structure-switching, hairpin probe, electrochemical, PDGF-BB
Abstract

Using platelet derived growth factor B chain (PDGF-BB) as the model analyte, a structure-switching hairpin probe based electrochemical biosensing strategy for highly sensitive detection of protein is proposed in this work. Aptamer probe hybridized with the immobilized hairpin structured signal probe to form DNA duplex. Upon binding with target protein, competition between target molecule and the signal probe with the aptamer probe happened, inducing the signal probe from stretched duplex to hairpin structure. Under optimum experimental conditions, the target protein can be sensitively detected in a linear dynamic range from 0.1 ng/mL to 500 ng/mL with a low detection limit of 0.08 ng/mL.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
October 2015
ISBN
978-94-6252-113-1
ISSN
2352-5401
DOI
https://doi.org/10.2991/icadme-15.2015.24How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Xia Hu
AU  - Song-Bai Zhang
AU  - Ji-Lin Lu
AU  - Li-Ying Zheng
AU  - Hong-En Geng
AU  - Yan-Qing Qiu
AU  - Yan-Yan Huang
AU  - Li-Ping Qiu
AU  - Yuan-Dao Chen
PY  - 2015/10
DA  - 2015/10
TI  - Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein
BT  - Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
PB  - Atlantis Press
SP  - 114
EP  - 117
SN  - 2352-5401
UR  - https://doi.org/10.2991/icadme-15.2015.24
DO  - https://doi.org/10.2991/icadme-15.2015.24
ID  - Hu2015/10
ER  -