Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

Intrinsic Fluorescence Spectra of Tryptophan, Tyrosine and Phenyloalanine

Authors
Hui Yang, Xue Xiao, Xuesong Zhao, Yan Wu
Corresponding Author
Hui Yang
Available Online October 2015.
DOI
10.2991/icadme-15.2015.46How to use a DOI?
Keywords
Fluorescence spectra, tryptophan, tyrosine, phenyloalanine
Abstract

This paper presents the intrinsic fluorescence spectra characteristics of tryptophan, tyrosine and phenyloalanine measured with 3D fluorescence spectrophotometer. Two strong fluorescence peaks of tryptophan locate at ex/ em=230/348nm and 280/348nm, three strong fluorescence peaks of tyrosine locate at ex/ em=202/304nm, ex/ em=220/304nm and 275/304nm, and two fluorescence peaks of phenyloalanine locate at ex/ em=210/280nm and ex/ em=260/280nm were found. The linear relationship of fluorescence intensity to solution concentration were also observed in condition of low solution concentration. The influence of pH of solution to the fluorescence intensity was also measured and discussed.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
October 2015
ISBN
10.2991/icadme-15.2015.46
ISSN
2352-5401
DOI
10.2991/icadme-15.2015.46How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Hui Yang
AU  - Xue Xiao
AU  - Xuesong Zhao
AU  - Yan Wu
PY  - 2015/10
DA  - 2015/10
TI  - Intrinsic Fluorescence Spectra of Tryptophan, Tyrosine and Phenyloalanine
BT  - Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
PB  - Atlantis Press
SP  - 224
EP  - 233
SN  - 2352-5401
UR  - https://doi.org/10.2991/icadme-15.2015.46
DO  - 10.2991/icadme-15.2015.46
ID  - Yang2015/10
ER  -