An E-DNA Sensor for Sequence-Specific DNA detection

DOI

10.2991/icadme-15.2015.330How to use a DOI?

Keywords

E-DNA sensor, MB, DNA

Abstract

In this work, a sensitive E-DNA sensor based on avidin modified electrode and DNA-functionalized Au nanoparticle (DFNP) was developed. The DNA-Functionalized Au nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’ terminal and a thiol at the 5’ terminal, the other is linearity signal DNA. Without hybridized with target DNA, the loop of hairpin impeded biotin linked with avidin on electrode. However, after target hybridization, hairpin was opened and biotin was recognized by avidin resulting in DNA-functionalized Au nanoparticle was brought on electrode surface. Electrochemical signals of methylene blue (MB) bound to the signal DNA were measured by differential pulse voltammetry (DPV). Introduction By using this new method, we demonstrate that this prototype sensor has been able to detect as low as picomolar DNA targets with excellent differentiation ability for even single mismatches.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Guobing Wei
AU  - Long Wan
AU  - Fusheng Liao
AU  - Hao Fan
PY  - 2015/10
DA  - 2015/10
TI  - An E-DNA Sensor for Sequence-Specific DNA detection
BT  - Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
PB  - Atlantis Press
SP  - 1794
EP  - 1797
SN  - 2352-5401
UR  - https://doi.org/10.2991/icadme-15.2015.330
DO  - 10.2991/icadme-15.2015.330
ID  - Wei2015/10
ER  -