Evaluation of Nitric Oxide Scavenging and Nitric Oxide Synthases Expression of Resveratrol and Polydatin

DOI

10.2991/aer.k.210817.003How to use a DOI?

Keywords

Nitric oxide, scavenging, Nitric oxide synthases, polydatin, Resveratrol

Abstract

Cellular damage induced by free-radicals like reactive oxygen species(ROS) has been implicated in several diseases. The ROS radicals like alkoxy radical(RO-) and peroxy radical (ROO-) are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events leading to DNA damage and cell death. Hence naturally anti-oxidant play a vital role in combating these conditions. Resveratrol (RES) and polydatin (PD) are two kinds of natural phytoalexin with excellent antimicrobial and anti-inflammatory activity. In this study aim to evaluation the anti-inflammatory ability of RES and PD on LPS-stimulate inflammatory cell model by Nitric oxide(NO) colorimetric method and NO synthases expression with qRT-PCR method, to provide a basis for the potential application and development in the future. The results showed that RES is significantly stronger than PD in NO synthases expression (P < 0.05). At the same time, the comparison of NO production inhibition rate between RES and PD are not significant (P > 0.05). RES and PD show their respective anti-inflammatory activity advantages under different indicators, which means we should choose RES or PD according to the different uses.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Wei-ding Wang
AU  - Zi-qi Lai
AU  - Zi-cong Wu
AU  - Yi-fei Wang
AU  - Zhi-ping Wang
PY  - 2021
DA  - 2021/08/18
TI  - Evaluation of Nitric Oxide Scavenging and Nitric Oxide Synthases Expression of Resveratrol and Polydatin
BT  - Proceedings of the 2021 10th International Conference on Applied Science, Engineering and Technology (ICASET 2021)
PB  - Atlantis Press
SP  - 11
EP  - 15
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.210817.003
DO  - 10.2991/aer.k.210817.003
ID  - Wang2021
ER  -