Cytotoxic Activity of Melinjo Seed Protein (Gnetum Gnemon L.) Against 4T1 Cells and Hela Cells, and Antiproliferation Test on 4T1 Cells

DOI

10.2991/978-94-6463-050-3_32How to use a DOI?

Keywords

Antiproliferation; Melinjo seed protein (Gnetum gnemon L.); cytotoxic; 4T1 cells; HeLa cells

Abstract

Melinjo (Gnetum gnemon L.) is an easily discovered plant in Indonesia. The previous researchers documented that the Melinjo seed has anticancer activity. This plant contains a high concentration of Ribosome Inactive Proteins (RIPs), which could inhibit protein synthesis. The research aims to determine the cytotoxic and antiproliferative activity of Melinjo seed extract against cervical cancer (HeLa) and breast cancer (4T1) cell lines. The extract was collected using the ion exchange DEAE matrix isolation method. The bioactivity test was done by MTT assay. The cytotoxic activity was determined by the IC₅₀ value of 361,1 µg/mL and 939,723 µg/mL against 4T1 and HeLa cells, respectively. The antiproliferative activity was determined by doubling the time value on the concentration of 15 µg/mL and 7,5 µg/mL at 24, 48, and 72 h of the test. The result showed no inhibition activity of the extract because the doubling time of the control cells group was higher than the tested group. It can be concluded that the protein fraction of Melinjo seed does not have the cytotoxic and antiproliferative activities against HeLa and 4T1 cell lines.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Kurnia Indah Fatmawati
AU  - Peni Indrayudha
AU  - Maryati
AU  - Azis Saifudin
AU  - Cita Hanif Muflihah
PY  - 2022
DA  - 2022/12/14
TI  - Cytotoxic Activity of Melinjo Seed Protein (Gnetum Gnemon L.) Against 4T1 Cells and Hela Cells, and Antiproliferation Test on 4T1 Cells
BT  - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
PB  - Atlantis Press
SP  - 370
EP  - 378
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-050-3_32
DO  - 10.2991/978-94-6463-050-3_32
ID  - Fatmawati2022
ER  -