Making Bacillus cereus Bacterial Growth Media from Soybeans (Glycine max (L.) Merril)

DOI

10.2991/978-94-6463-050-3_26How to use a DOI?

Keywords

Bacillus cereus; Soybean; Bacterial Media

Abstract

This study develops a selective medium for the growth of Bacillus cereus from soybeans, which can be directly applied in the laboratory to replace media made from meat extract. This study considers the economic aspect by making inexpensive microbiological media, as well as testing the halal aspect so that it can be applied to halal-certified industries. The sizes of soybean powder used were 44 mesh, 60 mesh, and 100 mesh. While the concentration of soybeans in the media 1, 2, 3, and 4 g/200 ml. The media is made by mixing soybean powder with the addition of mannitol, sodium chloride, phenol red, agar, and aquades. Media Mannitol Egg Yolk Polymyxin Agar is used to isolate Bacillus cereus bacteria, which is very widely used in industry and microbiology laboratories. Soybeans have a fairly cheap price compared to meat extract. Soybeans can be used as an alternative raw material for the manufacture of bacterial growth media. Soybeans are a source of high protein for the growth of Bacillus cereus bacteria, which can replace the presence of meat extract in standard media. The results showed that the optimal number of bacterial colonies of Bacillus cereus occurred at a concentration of 4 g/200 ml soybean powder and 100 mesh size of soybean powder with a maximum number of colonies of 8.67 CFU/ml. While the optimal bacterial colony size of Bacillus cereus occurred at a concentration of soy powder 4 g/200 ml, and soybean powder size 100 mesh with a maximum colony size of 15.74 mm. Soybeans can be used for the manufacture of halal and cheap Bacillus cereus bacterial growth media with quality equivalent to media made from meat extract.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Atmojo Sandi Utomo
AU  - Kun Harismah
AU  - Akida Mulyaningtyas
PY  - 2022
DA  - 2022/12/14
TI  - Making Bacillus cereus Bacterial Growth Media from Soybeans (Glycine max (L.) Merril)
BT  - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
PB  - Atlantis Press
SP  - 306
EP  - 317
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-050-3_26
DO  - 10.2991/978-94-6463-050-3_26
ID  - Utomo2022
ER  -