Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

Detection of Terpenoid and Flavonoid of Five Species of Mistletoes at Stelechocarpus burahol (Bl.) Hook.f. & Th and Lagerstroemia speciosa (L.) Pers. by using Thin Layer Chromatography Method

Authors
Djoko Santosa1, *, SM. Widyastuti2, Ummi Rosyidah2, Betha Silmia2
1Department of Pharmaceutical Biology, Faculty of Pharmacy,Universitas Gadjah Mada
2Department of Silviculture, Faculty of Forestry, Universitas Gadjah Mada
*Corresponding author. Email: djoko5346@ugm.ac.id
Corresponding Author
Djoko Santosa
Available Online 2 May 2022.
DOI
https://doi.org/10.2991/absr.k.220406.055How to use a DOI?
Keywords
Kepel; Bungur; Mistletoe; Thin Layer Chromatography; Terpenoid; Flavonoid; Quercitrin
Abstract

Mistletoe is a parasitic plant that is well known for causing significant damage to forestry plants; nevertheless, its value in traditional medicine has yet to be proven. This study aims to investigate terpenoid and flavonoid compounds of Macrosolen cochinchinensis (Lour.) Tiegh, Scurrula atropurpurea (Bl.) Dans., Scurrula ferruginea (Jack) Dans., Dendrophthoe pentandra (L.) Miq., Dendrophthoe falcata (L.f.) Ettingsh. found on Stelechocarpus burahol (Bl.) Hook.f. & Th, known as Kepel, and Lagerstroemia speciosa (L.) Pers, known as Bungur, using Thin Layer Chromatography (TLC). The research was conducted at Universitas Gadjah Mada’s Department of Pharmaceutical Biology. Each mistletoe was extracted using dichloromethane for terpenoid detection, and ethanol 70% for flavonoid detection. Terpenoid and flavonoid compounds were detected using Thin Layer Chromatography (TLC) in the stationary phase of silica gel 60 F254. The mobile phase of n-hexane: ethyl acetate (93:7) was used for terpenoid detection, meanwhile both mobile phase of n-butanol: ethyl acetate: formic acid: water (5:5:2:1) and ethyl acetate: formic acid: water (90:5:5) were used for flavonoid detection. The result was further investigated under visible light and UV light 254 & 366 nm followed by anisaldehyde-H2SO4 spray reagent and thymol standard for terpenoid detection, and sitroborat spray reagent with rutin, quercetin, and quercitrin standard for flavonoid detection. Terpenoid was found on hRf 10, 20, 50, 60, 70, and 85 whereas it was found on hRf 30 for thymol standard. Flavonoid was found on hRf: 40, 50, 60, 70, and 85 whereas it was found on hRf 60 for rutin standard. Quercetin was found in mistletoe and its host on hRf: 60, however, quercitrin was only found in mistletoe species on hRf: 65.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
978-94-6239-573-2
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.220406.055How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

TY  - CONF
AU  - Djoko Santosa
AU  - SM. Widyastuti
AU  - Ummi Rosyidah
AU  - Betha Silmia
PY  - 2022
DA  - 2022/05/02
TI  - Detection of Terpenoid and Flavonoid of Five Species of Mistletoes at Stelechocarpus burahol (Bl.) Hook.f. & Th and Lagerstroemia speciosa (L.) Pers. by using Thin Layer Chromatography Method
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 388
EP  - 393
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.055
DO  - https://doi.org/10.2991/absr.k.220406.055
ID  - Santosa2022
ER  -