Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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The Genetic Diversity of Moringa Oleifera on Poteran Island-Madura Based on Petiole Colors Using ISSR (Inter Simple Sequence Repeat) Method

Authors
Wirdhatul Muslihatin1, *, Triono Bagus Saputro1, Nur Isma Latifah1, Chusnul Eka Safitri Himayani1
1Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Jl. Teknik Mesin No. 173, Keputih, East Java, Indonesia.,
*Corresponding author. Email: wirdhabioits@gmail.com
Corresponding Author
Wirdhatul Muslihatin
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.006How to use a DOI?
Keywords
Genetic diversity; ISSR (inter simple sequence repeat); Moringa oleifera; Petiole colors
Abstract

Moringa oleifera, which is endemic of Pulau Poteran, Madura, is one of a genetic variation of Moringa. It is native Indonesian germplasm, which must be preserved to maintain the genetic biodiversity of Moringa. Known locally as kelor, the plant has four different color of petiole: red, white, purple, and green. The purpose of this study was to determine the genetic diversity of the plant with its four different colors of the petiole. This study used six ISSR molecular markers in the analysis. The stages of this research were the preparation of the leaf samples, the DNA extraction, the DNA quality and quantity observation, the PCR with ISSR (inter simple sequence repeat) primer, then the electrophoresis. The locus that shows bands was used for scoring: present (1) and is absent (0). Data analysis and the making of the dendrogram were done by using the unweighted pair group method arithmetic (UPGMA) via the MVSP program. The level of polymorphism generated by the ISSR marker was 97,2% with an average PIC of 0,46 and similarity value of 5%-66,7%.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
10.2991/absr.k.220406.006
ISSN
2468-5747
DOI
10.2991/absr.k.220406.006How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Wirdhatul Muslihatin
AU  - Triono Bagus Saputro
AU  - Nur Isma Latifah
AU  - Chusnul Eka Safitri Himayani
PY  - 2022
DA  - 2022/05/02
TI  - The Genetic Diversity of Moringa Oleifera on Poteran Island-Madura Based on Petiole Colors Using ISSR (Inter Simple Sequence Repeat) Method
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 39
EP  - 46
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.006
DO  - 10.2991/absr.k.220406.006
ID  - Muslihatin2022
ER  -