Antioxidant Activity Evaluation of Agarwood Aquilaria malaccensis Lamk. Leaves Extract Using DPPH, FRAP and ABTS Assays
- 10.2991/absr.k.220406.004How to use a DOI?
- Agarwood; Antioxidant; Aquilaria malaccensis Lamk.; Metabolite profiles
Indonesia has been known to have large forest areas and rich in biodiversity, which could be the source of primary and secondary metabolites. Along with the technological development and research in medicine, agarwood, which was initially used only for topical body treatments, room fragrances, and religious ceremonies, is started to be investigated for herbal medicines. This study aimed to perform metabolite profiling with spectrophotometry and evaluate the antioxidant activity in agarwood Aquilaria malaccensis Lamk using three assays: DPPH, FRAP and ABTS. The mature leaves used in this study were selected by their though leaf structure and dark green color. The leaves were dried and powdered before being extracted by soxhlet with three different solvents polarity i.e. ethanol, ethyl acetate, and n-hexane. Metabolite profiling was done by scanning the extracts from 200-800 nm. Antioxidant activities were analyzed using 2,2-diphenyl-1-picryhydrazil (DPPH), ethyl benzotiazolin sulfonate (ABTS) and Ferric Reducing Antioxidant Power (FRAP). The result showed that ethanol has the highest yield of extract, followed by ethyl acetate then n-hexane with 10.7%, 8.07%, and 5.23%, respectively. Ethanol contained the highest flavonoid compound compared to the others and has the highest antioxidant activity with IC50 of 37.22 µg/mL using DPPH assay, 66.33 µg/mL using ABTS and 220.188 ± 1.66 µmol TE/g sample using FRAP assays. Therefore, it can be concluded that ethanol extract of Aquilaria malaccensis Lamk. has the highest antioxidant activity and the richest flavonoid compounds compared to the others.
- © 2022 The Authors. Published by Atlantis Press International B.V.
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- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Aprilliani Prissilla Halim AU - Nastiti Wijayanti AU - Lisna Hidayati AU - Tri Rini Nuringtyas PY - 2022 DA - 2022/05/02 TI - Antioxidant Activity Evaluation of Agarwood Aquilaria malaccensis Lamk. Leaves Extract Using DPPH, FRAP and ABTS Assays BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 18 EP - 25 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.004 DO - 10.2991/absr.k.220406.004 ID - Halim2022 ER -