Preliminary Study: Bioautography Screening on Edible Local Plants with α-Glucosidase Inhibitor

DOI

10.2991/ahsr.k.211105.043How to use a DOI?

Keywords

diabetes mellitus type 2; TLC bioautography; rice; local vegetables

Abstract

Indonesia is ranked as one of the top ten countries with higher diabetes mellitus cases in 2019. In total, International Diabetes Federation recorded 10.7 million diabetes mellitus cases in 2019. Many researchers reported that diabetes mellitus type 2 could be controlled and managed with diet. Since local food is made from common plants that people can easily find, we collected edible local plats from a small village in Solok Regency and screened their inhibition toward enzyme α-glucosidase. Fourteen local edible plants were collected for the screening of α-glucosidase inhibitors. The plants were macerated with methanol to gain methanolic extract. The extracts were spotted at a concentration of 10 mg/mL with a volume of 8 μL on a silica TLC plat and then eluted with semi-polar mobile phase, chloroform: ethyl acetate: methanol = 65:20:15. Afterward, the TLC plate was sprayed with α-glucosidase inhibitor. The results showed that two samples were inhibited by α-glucosidase. They were rice (Oryza sativa L.) and torch ginger (Etlingera elatior (Jack) R.M.Sm.). The active spots in rice had rf values at 0.66 and 0.81, while torch ginger had rf values at 0.23, 0.25, and 0.63.

Copyright

© 2021 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Nova Syafni
AU  - Nurwahidatul Arifa
AU  - Friardi Ismed
AU  - Deddi Prima Putra
PY  - 2021
DA  - 2021/11/17
TI  - Preliminary Study: Bioautography Screening on Edible Local Plants with α-Glucosidase Inhibitor
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 295
EP  - 301
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.043
DO  - 10.2991/ahsr.k.211105.043
ID  - Syafni2021
ER  -