Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)

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Macroscopic and Microscopic Isolation and Identification of Cellulolytic Bacteria from Mixtures of Coal and Beef Cattle

Authors
Y. A. Hidayati1, *, M. R. Yusra1, G. C. K. Puteri1, E. T. Marlina1, D. Z. Badruzzaman1, E. Harlia1
1Indonesia Laboratory of Microbiology and Waste Management, Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, Indonesia
*Corresponding author. Email: yuli.astuti@unpad.ac.id
Corresponding Author
Y. A. Hidayati
Available Online 8 March 2023.
DOI
10.2991/978-94-6463-116-6_41How to use a DOI?
Keywords
Bacterial; Cellulolytic; Cellulolytic indexes; Coal; Feces
Abstract

Cellulolytic bacteria are bacteria that can hydrolyze cellulose into smaller polysaccharides glucose units. Glucose is used as a source of energy and carbon for bacterial growth. This study aimed to determine the ability of isolates bacteria from feces of beef cattle and coal (bituminous, sub-bituminous, lignite) to produce cellulase enzymes based on the clear zone seen surrounding the colonies and characterization of cellulolytic bacteria isolates from cattle feces and coal. The cellulolytic activity of the bacteria was determined by their ability to degrade nutrient-carboxymethyl cellulose (Na-CMC) substrates. The result showed that six isolates tested were able to produce cellulase. BC2 isolate bacteria had the highest cellulolytic activity, 1.23, based on 48-h production time. Macroscopic identification bacteria isolates showed that colonies formed white with a slimy structure, irregular shape, rounded, rhizoid, and wavy. Smooth, branched edges. Microscopic identification of bacteria isolates showed five bacteria isolates from beef cattle feces, and their combinations were gram-positive and bacil shape, two of the isolate’s bacteria coccus shape and one of which was a gram-negative group.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)
Series
Advances in Biological Sciences Research
Publication Date
8 March 2023
ISBN
10.2991/978-94-6463-116-6_41
ISSN
2468-5747
DOI
10.2991/978-94-6463-116-6_41How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Y. A. Hidayati
AU  - M. R. Yusra
AU  - G. C. K. Puteri
AU  - E. T. Marlina
AU  - D. Z. Badruzzaman
AU  - E. Harlia
PY  - 2023
DA  - 2023/03/08
TI  - Macroscopic and Microscopic Isolation and Identification of Cellulolytic Bacteria from Mixtures of Coal and Beef Cattle
BT  - Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)
PB  - Atlantis Press
SP  - 328
EP  - 333
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-116-6_41
DO  - 10.2991/978-94-6463-116-6_41
ID  - Hidayati2023
ER  -