Proceedings of the6th International Conference on Mechatronics, Materials, Biotechnology and Environment (ICMMBE 2016)

Effect on Hep-1cell Calcium Homeostasis after Aβ25-35 Injury

Authors
Wenzhao Liang, Bing Yan, Mingzhu Yu, Yankun Shao, Jinting He
Corresponding Author
Wenzhao Liang
Available Online September 2016.
DOI
https://doi.org/10.2991/icmmbe-16.2016.77How to use a DOI?
Keywords
Calcium Homeostasis; Aβ25-35; Alzheimer's disease
Abstract
The purpose of this study was to observe the effect of β-amyloid protein injury on intracellular calcium homeostasis in Hep-1 cells. Aβ25-35 was used to build the Hep-1 cell model of AD. Intracellular Calcium measurements was performed by incubation with the calcium indicator probe and then observed under laser confocal microscopy. Ca2+ release from intracellular stores was significantly increased in immortal human endothelial cells (SK-Hep-1) after injury induced by Aβ25-35. No significant difference was found in CCE from injured cells to controls. Increased β-amyloid protein production and intracellular calcium homeostasis disturbance were pathological mechanisms relate to AD.
Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)

Proceedings
6th International Conference on Mechatronics, Materials, Biotechnology and Environment (ICMMBE 2016)
Part of series
Advances in Engineering Research
Publication Date
September 2016
ISBN
978-94-6252-228-2
ISSN
2352-5401
DOI
https://doi.org/10.2991/icmmbe-16.2016.77How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Wenzhao Liang
AU  - Bing Yan
AU  - Mingzhu Yu
AU  - Yankun Shao
AU  - Jinting He
PY  - 2016/09
DA  - 2016/09
TI  - Effect on Hep-1cell Calcium Homeostasis after Aβ25-35 Injury
BT  - 6th International Conference on Mechatronics, Materials, Biotechnology and Environment (ICMMBE 2016)
PB  - Atlantis Press
SP  - 410
EP  - 413
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmmbe-16.2016.77
DO  - https://doi.org/10.2991/icmmbe-16.2016.77
ID  - Liang2016/09
ER  -