Effect of Bitter Extract (Andrographis paniculata Nees.) to Epididymal Mice Sperm Quality

DOI

10.2991/absr.k.200807.023How to use a DOI?

Keywords

andrographolide, bitter, epididymal, spermatozoa quality

Abstract

Bitter (Andrographis paniculata Nees.) used to prevention of various diseases, such as anticancer, anti- inflammatory, and antifertility. Ones of the bitter content is Andrographolide. It has antimitotic agent couse decreased of mitotic cells. The aimed of this research to determine the effects of Bitter extract to the mice (Mus musculus L. Swiss Webster)-epididymal sperm quality. The research used Completely Randomized Design (CRD), 4 treatments and 6 replications as the control (T0) without bitter extract treatment, and the other were gave a bitter extract orally for 36 days with dosages of T1 0,2; T2 0.4; or T3 0.6 g/ml/day. After treatment, the mice were decapitated, dissected and taken the epididymal. The number of normal or abnormal spermatozoa counted by a Neubauer Improved method and Eocine Stained. Data were Analyzed using Analysis of Varian and Duncan’s New Multiple Range Test. The results Showed the highest average number of sperm in T0 29.83 x 105, comparison to T1 18.17 x 105; T2 24.33 x 105 and T3 14.17 x 105. The avarage number of normal sperm increased as decreased of dosage. The number of abnormal sperm ncreased as the increased of dosage. It can conclude that the bitter extract Orally for 36 days can decrease of mice sperm quality and increase of abnormal sperm of mice.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ramadhan Sumarmin
AU  - Nadyatul Khaira Huda
AU  - Yusni Atifah
PY  - 2020
DA  - 2020/08/07
TI  - Effect of Bitter Extract (Andrographis paniculata Nees.) to Epididymal Mice Sperm Quality
BT  - Proceedings of the International Conference on Biology, Sciences and Education (ICoBioSE 2019)
PB  - Atlantis Press
SP  - 97
EP  - 100
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.200807.023
DO  - 10.2991/absr.k.200807.023
ID  - Sumarmin2020
ER  -