Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)

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Effect of Gamma-Ray Irradiation on Vetiver Grass (Vetiveria Zizanioides (L.) Nash.) in Vitro Shoots Growth and Multiplication

Authors
Wahyu Widoretno1, *, Miftahur Rohmah1, Serafinah Indriyani1
1Mathematics and Natural Sciences Faculty, Brawijaya University, Malang, Indonesia
*Corresponding author. Email: wahyu_widoretno@yahoo.com
Corresponding Author
Wahyu Widoretno
Available Online 22 May 2023.
DOI
10.2991/978-94-6463-166-1_26How to use a DOI?
Keywords
Gamma Irradiation; In Vitro Culture; Shoot Multiplication; Vetiveria zizanioides
Abstract

The vetiver plant, Vetiveria zizanioides (L.) Nash, yields vetiver oil, which is a fixative for perfumes. It is vital to create improved vetiver plants by in vitro mutagenesis because the low quality of vetiver in Indonesia causes vetiver oil productivity to decline year after year. This study sought to determine how gamma radiation affected the development and reproduction of vetiver in vitro shoots. Gamma radiation was used to irradiate the in vitro vetiver shoots at doses of 0; 15; 30; 45; 60; and 75 Gy. The irradiated shoot explants were subsequently cultivated on medium containing MS + NAA 0.1 mg/L + BAP 1 mg/L during eight weeks. The outcomes demonstrated that in vitro shoot growth and multiplication were reduced by gamma radiation. With 50 shoots per explant, the percentage of explants generating shoots without radiation and with a low dose of 15 Gy was 100%. When exposed to moderate doses of 30–45 Gy, 60–68 percent of the explants survived and produced 13–25 shoots per explant. Less than 50% of explants survived high dosages of 60–75%, and less than 30% of explants developed shoots with fewer than 10 shoots per explant. The gamma-ray irradiation lethal dosage (LD50) for the survival rate of the shoots was 61 Gy.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 May 2023
ISBN
10.2991/978-94-6463-166-1_26
ISSN
2468-5747
DOI
10.2991/978-94-6463-166-1_26How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Wahyu Widoretno
AU  - Miftahur Rohmah
AU  - Serafinah Indriyani
PY  - 2023
DA  - 2023/05/22
TI  - Effect of Gamma-Ray Irradiation on Vetiver Grass (Vetiveria Zizanioides (L.) Nash.) in Vitro Shoots Growth and Multiplication
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 181
EP  - 190
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_26
DO  - 10.2991/978-94-6463-166-1_26
ID  - Widoretno2023
ER  -