Fish Biodiversity Monitoring in Singkarak Lake, West Sumatra: Comparison of Fish Detections Using Environmental DNA and Conventional Methods

DOI

10.2991/978-94-6463-166-1_56How to use a DOI?

Keywords

Singkarak lake; monitoring; environmental DNA; Next generation sequencing; specific primer

Abstract

Singkarak lake is the largest lake in West Sumatra that is experiencing a decline in fish species. The main factors are constructing hydroelectric power plants, degradation, overfishing, and invasive species. Therefore, a reliable non-invasive survey method is needed to detect fish species. A monitoring study was conducted to compare the conventional recording of Singkarak Lake fish biodiversity with the eDNA method. Water samples were taken as much as one L with two replications to filtering and amplified using universal fish primers with the NGS technique. The study detected as many as 147 species from 80 genera, 18 families, and nine orders. The study detected ten species of the total (30) previously reported in Singkarak Lake. Four species were found in all studies: Barbonymus schwanefeldii, Clarias batrachus, Mystacoleucus padangensis, and Osteochilus vittatus/Osteochilus hasseltii. The study also detected the species that have never been previously reported in Singkarak Lake (135 species). The study showed the eDNA method could be used for fish monitoring by considering some factors: DNA quality, contamination, the use of specific primers, and the availability of sequence in Genbank. The results can be useful for biomonitoring the other taxa in Singkarak Lake, West Sumatra, using the eDNA method.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - D. I. Roesma
AU  - D. H. Tjong
AU  - Syaifullah
AU  - Nofrita
AU  - M. N. Janra
AU  - F. D. L. Prawira
AU  - V. M. Salis
AU  - D. R. Aidil
PY  - 2023
DA  - 2023/05/22
TI  - Fish Biodiversity Monitoring in Singkarak Lake, West Sumatra: Comparison of Fish Detections Using Environmental DNA and Conventional Methods
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 461
EP  - 474
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_56
DO  - 10.2991/978-94-6463-166-1_56
ID  - Roesma2023
ER  -